Human Amphiregulin Biotinylated Antibody Summary
Val107-Lys184
Accession # P15514
Applications
Human Amphiregulin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Amphiregulin
Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.
Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5 - 10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.
Product Datasheets
Citations for Human Amphiregulin Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces
Authors: AM Tsou, H Yano, CN Parkhurst, T Mahlakõiv, C Chu, W Zhang, Z He, KJ Jarick, C Zhong, GG Putzel, M Hatazaki, JRI IBD Li, IC Lorenz, D Andrew, P Balderes, CSN Klose, SA Lira, D Artis
Nature, 2022-11-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tissue remodeling by an opportunistic pathogen triggers allergic inflammation
Authors: Karen Agaronyan, Lokesh Sharma, Bharat Vaidyanathan, Keith Glenn, Shuang Yu, Charles Annicelli et al.
Immunity
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Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response
Authors: LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto
Sci Rep, 2019-06-18;9(1):8702.
Species: Human
Sample Types: Serum
Applications: ELISA Detection -
ADAM17 substrate release in proximal tubule drives kidney fibrosis
JCI Insight, 2016-08-18;1(13):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.
Authors: Stoll S, Stuart P, Lambert S, Gandarillas A, Rittie L, Johnston A, Elder J
J Invest Dermatol, 2016-02-01;136(2):444-52.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development -
Regulation of Amphiregulin Gene Expression by beta -Catenin Signaling in Human Hepatocellular Carcinoma Cells: A Novel Crosstalk between FGF19 and the EGFR System
Authors: Maria U. Latasa, Fabiana Salis, Raquel Urtasun, Oihane Garcia-Irigoyen, Maria Elizalde, Iker Uriarte et al.
PLoS ONE
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TACE/ADAM-17 Phosphorylation by PKC-Epsilon Mediates Premalignant Changes in Tobacco Smoke-Exposed Lung Cells
Authors: Hassan Lemjabbar-Alaoui, Sukhvinder S. Sidhu, Aklilu Mengistab, Marianne Gallup, Carol Basbaum
PLoS ONE
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Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development
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