Home / Alkaline Phosphatase/ALPL / Human Alkaline Phosphatase/ALPL PE-conjugated Antibody

Human Alkaline Phosphatase/ALPL PE-conjugated Antibody

Catalog #: FAB1448P
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1448P-100
FAB1448P-025
Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Alkaline Phosphatase/ALPL PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
Monoclonal Mouse IgG1 Clone # B4-78
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL PE-conjugated Monoclonal Antibody (Catalog # FAB1448P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/ALPL PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Human osteoblasts obtained from distinct periarticular sites demonstrate differences in biological function in vitro
    Authors: Erden Ali, Mark Birch, Niina Hopper, Neil Rushton, Andrew W. McCaskie, Roger A. Brooks
    Bone & Joint Research
  2. Osteogenic potential of adipogenic predifferentiated human bone marrow‐derived multipotent stromal cells for bone tissue‐engineering
    Authors: Adrien Moya, Nathanaël Larochette, Marianne Bourguignon, Hanane El‐Hafci, Esther Potier, Hervé Petite et al.
    Journal of Tissue Engineering and Regenerative Medicine
  3. Single cell gene expression profiling of cortical osteoblast lineage cells
    Authors: James M. Flynn, Steven C. Spusta, Clifford J. Rosen, Simon Melov
    Bone
  4. Botulinum Toxin Treatment of Adult Muscle Stem Cells from Children with Cerebral Palsy and hiPSC-Derived Neuromuscular Junctions
    Authors: Costamagna, D;Bastianini, V;Corvelyn, M;Duelen, R;Deschrevel, J;De Beukelaer, N;De Houwer, H;Sampaolesi, M;Gayan-Ramirez, G;Campenhout, AV;Desloovere, K;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Muscle Microbiopsy to Delineate Stem Cell Involvement in Young Patients: A Novel Approach for Children With Cerebral Palsy
    Authors: M Corvelyn, N De Beukela, R Duelen, J Deschrevel, A Van Campen, S Prinsen, G Gayan-Rami, K Maes, G Weide, K Desloovere, M Sampaolesi, D Costamagna
    Front Physiol, 2020-08-06;11(0):945.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Interstitial Cell Remodeling Promotes Aberrant Adipogenesis in Dystrophic Muscles
    Authors: J Camps, N Breuls, A Sifrim, N Giarratana, M Corvelyn, L Danti, H Grosemans, S Vanuytven, I Thiry, M Belicchi, M Meregalli, K Platko, ME MacDonald, RC Austin, R Gijsbers, G Cossu, Y Torrente, T Voet, M Sampaolesi
    Cell Rep, 2020-05-05;31(5):107597.
    Species: Human
    Sample Types: Whole Cells
  7. Intrinsic Myogenic Potential of Skeletal Muscle-Derived Pericytes from Patients with Myotonic Dystrophy Type 1
    Authors: CRM Ausems, RHL Raaijmaker, WJAA van den Br, M Willemse, BGM van Engele, DG Wansink, H van Bokhov
    Mol Ther Methods Clin Dev, 2019-09-12;15(0):120-132.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia
    Authors: W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin
    Theranostics, 2018-11-09;8(20):5575-5592.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration
    Authors: MGMC Mori da Cu, G Giacomazzi, G Callewaert, L Hympanova, F Russo, G Vande Veld, R Gijsbers, M Albersen, M Sampaolesi, J Deprest
    Sci Rep, 2018-07-13;8(1):10604.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  10. A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.
    Authors: Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T
    PLoS ONE, 2014-06-09;9(6):e99534.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Leukocyte alkaline phosphatase (LAP) by flow cytometry.
    Authors: Eshel, Elizabet, Sharabi-Nov, Adi, Dally, Najib
    MLO Med Lab Obs, 2013-08-01;45(8):22, 24, 26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
    Stem Cells, 2011-09-01;29(9):1469-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Stem cells from deciduous tooth repair mandibular defect in swine.
    Authors: Zheng Y, Liu Y, Zhang CM
    J. Dent. Res., 2009-03-01;88(3):249-54.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Mesodermal iPSC–derived progenitor cells functionally regenerate cardiac and skeletal muscle
    Authors: Mattia Quattrocelli, Melissa Swinnen, Giorgia Giacomazzi, Jordi Camps, Ines Barthélemy, Gabriele Ceccarelli et al.
    Journal of Clinical Investigation
  15. Attenuating iPSC reprogramming stress with dominant-negative BET peptides
    Authors: Md Emon Hossain, Ricardo Raul Cevallos, Ruowen Zhang, Kejin Hu
    iScience

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