Human Akt2 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC23152R-100UG
R&D Systems Antibodies
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Human Akt2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Akt2. Using direct ELISA, this antibody does not detect recombinant human (rh) Akt1 or rhAkt3.
Source
Monoclonal Mouse IgG1 Clone # 302501
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2
Asn2-Glu481
Accession # P31751
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Akt2

The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 2
Entrez Gene IDs
208 (Human); 11652 (Mouse); 29414 (Rat)
Alternate Names
Akt2; EC 2.7.11; EC 2.7.11.1; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-beta; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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