Human AgRP/ART Antibody

Catalog # Availability Size / Price Qty
MAB8311
MAB8311-SP
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Human AgRP/ART Antibody Summary

Species Reactivity
Human
Specificity
Detects human AgRP in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse AgRP.
Source
Monoclonal Mouse IgG2B Clone # 82806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP
Ala21-Thr132
Accession # O00253
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human AgRP/ART (Catalog # 704-AG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

AgRP, also known as the protein product of the Agouti related transcript (ART), functions as an antagonist of the melanocortin (MC-3 and MC-4) receptors, both of which are implicated in obesity. AgRP levels are significantly elevated in the serum of obese mice and people.

Long Name
Agouti-related Protein
Entrez Gene IDs
181 (Human); 11604 (Mouse)
Alternate Names
agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2

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