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Human AgRP/ART Antibody

Catalog #: MAB8311 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8311
MAB8311-SP
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Human AgRP/ART Antibody Summary

Species Reactivity
Human
Specificity
Detects human AgRP in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse AgRP.
Source
Monoclonal Mouse IgG2B Clone # 82806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP
Ala21-Thr132
Accession # O00253
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human AgRP/ART (Catalog # 704-AG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

AgRP, also known as the protein product of the Agouti related transcript (ART), functions as an antagonist of the melanocortin (MC-3 and MC-4) receptors, both of which are implicated in obesity. AgRP levels are significantly elevated in the serum of obese mice and people.

Long Name
Agouti-related Protein
Entrez Gene IDs
181 (Human); 11604 (Mouse)
Alternate Names
agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2

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