Human Adiponutrin/PNPLA3 Antibody Summary
Arg160-Arg349
Accession # Q9NST1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Adiponutrin/ADPN in Human Adipocytes. Adiponutrin/ADPN was detected in immersion fixed human adipocytes using 10 µg/mL Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Adiponutrin/ADPN by Western Blot. Western blot shows lysates of human adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/ADPN at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponutrin/PNPLA3
Human Adiponutrin (ADPN; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10‑179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42‑62. There appear to be two splice variants. One shows a four aa deletion of aa 59‑62 and a second shows an alternate start site at Met379. Over aa 160‑349, human ADPN shares 61% aa identity with mouse Adiponutrin.
Product Datasheets
Citations for Human Adiponutrin/PNPLA3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis
Authors: D Hendriks, JF Brouwers, K Hamer, MH Geurts, L Luciana, S Massalini, C López-Igle, PJ Peters, MJ Rodríguez-, S Chuva de S, B Artegiani, H Clevers
Nature Biotechnology, 2023-02-23;0(0):.
Species: Human
Sample Types: Organoid
Applications: ICC -
Hepatic patatin‐like phospholipase domain‐containing 3 levels are increased in I148M risk allele carriers and correlate with NAFLD in humans
Authors: Elke Ericson, Linnéa Bergenholm, Anne‐Christine Andréasson, Carly I. Dix, Jane Knöchel, Sara F. Hansson et al.
Hepatology Communications
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