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Human ADAM10 Ectodomain APC-conjugated Antibody

Catalog #: IC1427A
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1427A
Detection of ADAM10 in MCF‑7 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM10 Ectodomain APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAM10 in direct ELISAs and Western blots. In Western blots, shows less than 5% cross-reactivity with recombinant human (rh) ADAM8, 9, 15, 17/TACE, and recombinant mouse ADAM10.
Source
Monoclonal Mouse IgG2B Clone # 163003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAM10
Thr214-Glu672
Accession # O14672
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of ADAM10 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ADAM10 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ADAM10 Ectodomain APC-conjugated Monoclonal Antibody (Catalog # IC1427A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

References
  1. Rooke, et al. (1996) Science 273:1227.
  2. Pan and Rubin (1997) Cell 90:271.
  3. Qi, et al. (1999) Science 283:91.
  4. Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry and Glynn (1990) Biochem. J. 268:245.
  9. Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs
102 (Human); 11487 (Mouse)
Alternate Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease

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Citations for Human ADAM10 Ectodomain APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
    Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
    Cellular Signalling, 2022-05-21;96(0):110360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.
    Authors: Endsley M, Somasunderam A, Li G, Oezguen N, Thiviyanathan V, Murray J, Rubin D, Hodge T, O'Brien W, Lewis B, Ferguson M
    Virology, 2014-02-22;454(0):60-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC-Fr

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