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Human Acylated Ghrelin Antibody

Catalog #: MAB8149 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8149
MAB8149-SP
Acylated Ghrelin in Human Small Intestine Tissue.
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Human Acylated Ghrelin Antibody Summary

Species Reactivity
Human
Specificity
Detects only the acylated form of human Ghrelin in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 891240
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Ghrelin acylated peptide
Gly24-Arg51
Accession # Q9UBU3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Acylated Ghrelin antibody in Human Small Intestine Tissue by Immunohistochemistry (IHC-P). View Larger

Acylated Ghrelin in Human Small Intestine Tissue. Acylated Ghrelin was detected in immersion fixed paraffin-embedded sections of human small intestine tissue using Mouse Anti-Human Acylated Ghrelin Monoclonal Antibody (Catalog # MAB8149) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ghrelin

Mature Ghrelin peptides are the result of the clavage of the Ghrelin/Obestatin Prepropeptide, a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98).  The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases.  Mature Ghrelin is acylated with an N-octanoyl group on serine 3 which is required for receptor binding.  Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa.  Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs.  Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.

Entrez Gene IDs
51738 (Human); 58991 (Mouse); 59301 (Rat)
Alternate Names
appetite-regulating hormone; Ghrelin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; GHRL; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related Peptide; MTLRPgrowth hormone secretagogue receptor ligand; obestatin; Protein M46

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