Home / Activin RIIA / Human Activin RIIA Antibody

Human Activin RIIA Antibody

Catalog #: AF340
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF340
AF340-SP
Activin RIIA in Human Prostate Cancer Tissue.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Activin RIIA Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin RIIA in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human Activin RIIB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIIA
Ser25-Asp82
Accession # P27037
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Activin RIIA Fc Chimera (Catalog # 340‑R2)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of biotinylated Recombinant Human/Mouse/Rat Activin A to immobilized Recombinant Human RIIA Fc Chimera (Catalog # 340‑R2) coated at 500 ng/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Activin RIIA antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Activin RIIA in Human Prostate Cancer Tissue. Activin RIIA was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human Activin RIIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF340) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIIA

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

References
  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology, 55:953.
Long Name
Activin Receptor IIA
Entrez Gene IDs
92 (Human); 11480 (Mouse)
Alternate Names
activin A receptor, type II; activin A receptor, type IIA; Activin receptor type IIA; activin receptor type-2A; Activin RIIA; ActivinRIIA; ACTRII; ACTRIIA; ACVR2A; ACVR2ACTR-IIA; AVR2A; EC 2.7.11; EC 2.7.11.30

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Citations for Human Activin RIIA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Activin receptors regulate the oligodendrocyte lineage in health and disease
    Authors: A Dillenburg, G Ireland, RK Holloway, CL Davies, FL Evans, M Swire, ME Bechler, D Soong, TJ Yuen, GH Su, JC Becher, C Smith, A Williams, VE Miron
    Acta Neuropathol., 2018-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination
    Authors: Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al.
    Nature Neuroscience
  3. Treatment with a soluble receptor for activin improves bone mass and structure in the axial and appendicular skeleton of female cynomolgus macaques (Macaca fascicularis).
    Authors: Fajardo RJ, Manoharan RK, Pearsall RS, Davies MV, Marvell T, Monnell TE, Ucran JA, Pearsall AE, Khanzode D, Kumar R, Underwood KW, Roberts B, Seehra J, Bouxsein ML
    Bone, 2009-09-23;46(1):64-71.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Plasma
    Applications: ELISA Development
  4. Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
    Authors: Bristol-Gould SK, Kreeger PK, Selkirk CG, Kilen SM, Cook RW, Kipp JL, Shea LD, Mayo KE, Woodruff TK
    Dev. Biol., 2006-06-17;298(1):132-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
    Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
    Dev. Biol., 2006-04-27;295(2):473-85.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary.
    Authors: Bristol SK, Woodruff TK
    Biol. Reprod., 2003-12-03;70(3):846-59.
    Species: Feline
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Regulation of endocytosis of activin type II receptors by a novel PDZ protein through Ral/Ral-binding protein 1-dependent pathway.
    Authors: Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, Tsuchida K, Sugino H
    J. Biol. Chem., 2002-03-06;277(21):19008-18.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation

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