Human AATK Antibody

Catalog #: MAB4866 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4866
MAB4866-SP

Detection of AATK in A172 Human Cell Line by Flow Cytometry.

1 Image

All AATK Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AATK Antibody Summary

Species Reactivity

Human

Specificity

Detects human AATK in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 456006

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human AATK
Asn421-Ala589
Accession # AAH47378

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of AATK antibody in A172 Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of AATK in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human AATK Monoclonal Antibody (Catalog # MAB4866, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AATK

Apoptosis-associated tyrosine kinase (AATK) is a 155 kDa membrane-associated intracellular protein that promotes neurite extension and neuronal differentiation. Low extracellular potassium levels induce AATK phosphorylation and apoptosis in cerebellar granule cells. AATK negatively regulates the Na-K-2Cl cotransporter by promoting its PP1-dependent dephosphorylation. The two alternate splice forms of AATK also contain the sequences included in this immunogen. Within this region, human AATK shares 63% aa sequence identity with mouse AATK.

Long Name

Apoptosis-associated Tyrosine Kinase

Entrez Gene IDs

9625 (Human); 11302 (Mouse); 690853 (Rat)

Alternate Names

AATK; AATYK; AATYKp35-binding protein; apoptosis-associated tyrosine kinaseCDK5-binding protein; Brain apoptosis-associated tyrosine kinase; EC 2.7.11; KIAA0641p35BP; Lemur tyrosine kinase 1; LMR1; LMR1EC 2.7.11.1; LMTK1; LMTK1AATYK1; p35BP; serine/threonine-protein kinase LMTK1