Human A20/TNFAIP3 Antibody Summary
Lys91-Leu263
Accession # P21580
Applications
Immersion fixedparaffin-embedded sections of normal breast and normal liver
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
A20/TNFAIP3 in HL‑60 Human Cell Line. A20/TNFAIP3 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
View Larger
Detection of A20/TNFAIP3 in Normal Breast. A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of normal breast using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
View Larger
Detection of A20/TNFAIP3 in Normal Liver. A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of normal liver using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: A20/TNFAIP3
A20, also called TNF alpha -induced protein 3 (TNFAIP3), is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human A20/TNFAIP3 Antibody
There are currently no reviews for this product. Be the first to review Human A20/TNFAIP3 Antibody and earn rewards!
Have you used Human A20/TNFAIP3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
