Human 5-HT2A Antibody Summary
Accession # P28223
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![](https://resources.rndsystems.com/images/datasheets/mab11250_human-5-ht2a-mab-clone-1055217-western-blot-1992022111958.jpg)
Detection of Human 5-HT Transporter Compounds by Western Blot. Western blot shows lysates of Human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human 5-HT Transporter Compounds Monoclonal Antibody (Catalog # MAB11250) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 5-HT Transporter Compounds at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
![](https://resources.rndsystems.com/images/datasheets/mab11250_human-5-ht2a-mab-clone-1055217-immunocytochemistry-2392022145211.jpg)
Detection of 5-HT Transporter Compounds in Transfected NSO (positive) and Wild Type NSO (negative). 5-HT Transporter Compounds was detected in immersion fixed transfected NSO (positive) and wild type NSO (negative) using Mouse Anti-Human 5-HT Transporter Compounds Monoclonal Antibody (Catalog # MAB11250) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
![](https://resources.rndsystems.com/images/datasheets/mab11250_human-5-ht2a-mab-clone-1055217-flow-cytometry-239202216035.jpg)
Detection of 5-HT2A in HEK293 Human Cell Line Transfected with Human 5-HT2A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human 5-HT2A and eGFP was stained with (A) Mouse Anti-Human 5-HT2A Monoclonal Antibody (Catalog # MAB11250) or (B) Mouse IgG1 control antibody staining (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5-HT2A
Human serotonin receptor HTR2A, also known as 5-HT2A or HTR2, is a G-protein coupled receptor (GPCR) for 5-hydroxytryptamine (serotonin). The 5-HT2A receptor is mainly a cell surface receptor with several intracellular locations. Human -5-HT2A is the main excitatory receptor subtype among the GPCRs for serotonin but an inhibitory effect on the visual cortex and the orbitofrontal cortex has also been described. This receptor was first noted for its importance as a target of serotonergic psychedelic drugs such as LSD and psilocybin mushrooms.
Product Datasheets
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