Human 4-1BB/TNFRSF9/CD137 Antibody

Catalog #: MAB8382 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8382-100
MAB8382-SP

Detection of 4-1BB/TNFRSF9/CD137 in Human PHA-treated PBMC by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 4-1BB/TNFRSF9/CD137 Antibody Summary

Specificity

Detects human 4-1BB/TNFRSF9/CD137 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2356C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human 4-1BB/TNFRSF9/CD137
Leu24-His183
Accession # Q07011

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Agonist Activity

Measured by its ability to co-stimulate IL-2 secretion by human T cells in the presence of anti-CD3. The ED₅₀ for this effect is typically 1-10 μg/mL.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 4-1BB/TNFRSF9/CD137 in Human PHA-treated PBMC by Flow Cytometry. Human PBMC treated with PHA for 48 hr were stained with (A) Rabbit Anti-Human 4-1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB8382) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Goat-anti Rabbit IgG secondary antibody (Catalog # F0111) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Agonist Activity

Human 4-1BB/TNFRSF9/CD137 Antibody Stimulates IL-2 Secretion antibody in Human T Cells.

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Human 4-1BB/TNFRSF9/CD137 Antibody Stimulates IL-2 Secretion in Human T Cells. Rabbit Anti-Human 4-1BB/TNFRSF9/CD137 Monoclonal Antibody (Catalog # MAB8382) co-stimulates IL-2 secretion in human T cells in the presence of anti-CD3 in a dose-dependent manner, as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 1-10 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine-rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain (2, 3). Within the ECD, human 4-1BB shares 60% aa sequence identity with mouse and rat 4-1BB. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4⁺, CD8⁺, memory CD8⁺, NKT, and regulatory T cells (4-7) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (8-11). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (3, 8). This interaction costimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-7). It can also enhance the activation-induced cell death of repetitively stimulated T cells (3). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8⁺ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (8, 11) but supports mature dendritic cell survival and costimulates the proliferation and activation of mast cells (9, 10).
4-1BB activation enhances CD8⁺ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17).

References

Wang, C. et al. (2009) Immunol. Rev. 229:192.
Schwarz, H. et al. (1993) Gene 134:295.
Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
Wen, T. et al. (2002) J. Immunol. 168:4897.
Pulle, G. et al. (2006) J. Immunol. 176:2739.
Zheng, G. et al. (2004) J. Immunol. 173:2428.
Kim, D. et al. (2008) J. Immunol. 180:2062.
Lee, S. et al. (2008) Nat. Immunol. 9:917.
Choi, B.K. et al. (2009) J. Immunol. 182:4107.
Nishimoto, H. et al. (2005) Blood 106:4241.
Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
Lee, S. et al. (2005) J. Immunol. 174:6803.
Ma, B.Y. et al. (2005) Blood 106:2002.
Choi, B.K. et al. (2010) J. Immunol. 185:1404.
Kim, C. et al. (2011) Diabetes 60:3159.
Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
Salih, H.R. et al. (2001) J. Immunol. 167:4059.

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Alternate Names

4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9