HM Janelia Fluor® 526, NHS ester
Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
Purity: ≥90%
Biological Activity
Key information: HM Janelia Fluor® 526, NHS ester is a fluorogenic spontaneously blinking yellow-emitting dye; supplied with an NHS ester reactive group for the labeling of primary amines; hydroxymethyl derivative of JF526. Suitable for live cell imaging.Application: Suitable for confocal microscopy, super-resolution microscopy (SRM) including dSTORM, STED, SIM and single-molecule localization spectroscopy (SMLSM). HM Janelia Fluor® 526, NHS ester is cell permeable.
Properties and Photophysical Data: The spontaneous blinking properties of HM Janelia Fluor® 526, NHS ester avoids the requirement for strongly reducing dSTORM buffers. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was HM Janelia Fluor® 526, SE.
Optical Data for HM Janelia Fluor® 526, NHS ester
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | dSTORM, SMLSM, STED, SIM, Confocal Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging.
Zheng et al.
ACS Cent.Sci., 2019;5:1602
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