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His Tag Antibody

Catalog #: MAB050
98 Citations 14 Reviews Datasheet / COA / SDS
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MAB050-SP
MAB050-100
MAB050-500
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Detection of His Tag by Western Blot.
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Product Details
Citations (98)
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Reviews (14)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

His Tag Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunoaffinity Purification
Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of His Tag antibody by Western Blot. View Larger

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Proteins antibody by Flow Cytometry. View Larger

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Flow Cytometry View Larger

SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody. Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (IC050A, filled histogram). No staining was observed in the absence of protein (open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Simple Western Detection of His Tag antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of CHO Chinese hamster ovary cell line transfected with His-tagged human S1P4/EDG-6, loaded at 0.5 mg/mL. A specific band was detected for His-tagged S1P4/EDG-6 at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; His6-Tag; polyhistidine tag; polyHistidine; Poly-histidine

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Citations for His Tag Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

98 Citations: Showing 1 - 10
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  1. Anti-latent TGF beta binding protein 4 antibody improves muscle function and reduces muscle fibrosis in muscular dystrophy
    Authors: Alexis R. Demonbreun, Katherine S. Fallon, Claire C. Oosterbaan, Lauren A. Vaught, Nina L. Reiser, Elena Bogdanovic et al.
    Science Translational Medicine
  2. Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense.
    Authors: Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B
    Front Immunol, 2022-09-29;13(0):933329.
  3. Recombinant annexin A6 promotes membrane repair and protects against muscle injury
    Authors: Alexis R. Demonbreun, Katherine S. Fallon, Claire C. Oosterbaan, Elena Bogdanovic, James L. Warner, Jordan J. Sell et al.
    Journal of Clinical Investigation
  4. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation
    Authors: Xiang Xiao, Xiaomin Shi, Yihui Fan, Xiaolong Zhang, Minhao Wu, Peixiang Lan et al.
    Nature Communications
  5. Rational engineering of an improved adenosine deaminase 2 enzyme for weaponizing T-cell therapies
    Authors: J.R. Cox, M. Jennings, C. Lenahan, M. Manion, S. Courville, J. Blazeck
    Immuno-Oncology and Technology
  6. A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme
    Authors: Gilli Galore-Haskel, Yael Nemlich, Eyal Greenberg, Shira Ashkenazi, Motti Hakim, Orit Itzhaki et al.
    Oncotarget
  7. Insights into the Autoproteolytic Processing and Catalytic Mechanism of the Chlamydia trachomatis Virulence-Associated Protease CPAF
    Authors: Brianne M. Dudiak, Kenneth R. Maksimchuk, Maria M. Bednar, Christopher J. Podracky, Jonathan M. Burg, Tri M. Nguyen et al.
    Biochemistry
  8. Deep Mining of Complex Antibody Phage Pools Generated by Cell Panning Enables Discovery of Rare Antibodies Binding New Targets and Epitopes
    Authors: Anne Ljungars, Carolin Svensson, Anders Carlsson, Elin Birgersson, Ulla-Carin Tornberg, Björn Frendéus et al.
    Frontiers in Pharmacology
  9. Soluble markers of B cell activation suggest a role of B cells in the pathogenesis of systemic sclerosis-associated pulmonary arterial hypertension
    Authors: Sébastien Sanges, Thomas Guerrier, Alain Duhamel, Lucile Guilbert, Carine Hauspie, Alexis Largy et al.
    Frontiers in Immunology
  10. Cell targeting and immunostimulatory properties of a novel Fc gamma -receptor-independent agonistic anti-CD40 antibody in rhesus macaques
    Authors: Xianglei Yan, Sebastian Ols, Rodrigo Arcoverde Cerveira, Klara Lenart, Fredrika Hellgren, Kewei Ye et al.
    Cellular and Molecular Life Sciences
  11. Preparation and application of a novel monoclonal antibody specific for human B7-H3
    Authors: JIAN SHI, DONG-LEI ZHANG, ZHI-CHU CUI, HUI-MIN WANG
    Molecular Medicine Reports
  12. Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis
    Authors: Peter S. Back, William J. O’Shaughnessy, Andy S. Moon, Pravin S. Dewangan, Xiaoyu Hu, Jihui Sha et al.
    Proceedings of the National Academy of Sciences
  13. Modelling the structure of Short Gastrulation and generation of a toolkit for studying its function in Drosophila
    Authors: Sophie L. Frampton, Catherine Sutcliffe, Clair Baldock, Hilary L. Ashe
    Biology Open
  14. Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation
    Authors: Bei Yang, Goran Stjepanovic, Qingtao Shen, Andreas Martin, James H. Hurley
    Nature Structural & Molecular Biology
  15. Thyroid hormone receptor-beta 1 signaling is critically involved in regulating secondary ossification via promoting transcription of the Ihh gene in the epiphysis
    Authors: Weirong Xing, Patrick Aghajanian, Helen Goodluck, Chandrasekhar Kesavan, Shaohong Cheng, Sheila Pourteymoor et al.
    American Journal of Physiology-Endocrinology and Metabolism
  16. The Vaccinia Virus H3 Envelope Protein, a Major Target of Neutralizing Antibodies, Exhibits a Glycosyltransferase Fold and Binds UDP-Glucose
    Authors: Kavita Singh, Apostolos G. Gittis, Rossitza K. Gitti, Stanley A. Ostazeski, Hua-Poo Su, David N. Garboczi
    Journal of Virology
  17. Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs
    Authors: Lin Lei, Karen Tran, Yimeng Wang, James J. Steinhardt, Yongli Xiao, Chi-I Chiang et al.
    Frontiers in Microbiology
  18. Discovery of Compounds Inhibiting the ADP-Ribosyltransferase Activity of Pertussis Toxin
    Authors: Yashwanth Ashok, Moona Miettinen, Danilo Kimio Hirabae de Oliveira, Mahlet Z. Tamirat, Katja Näreoja, Avlokita Tiwari et al.
    ACS Infectious Diseases
  19. Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Authors: Dong Zhang, Feng Jiang, Rinat Zaynagetdinov, Hui Huang, Vanita D. Sood, Hong Wang et al.
    OncoImmunology
  20. Adamts10 inactivation in mice leads to persistence of ocular microfibrils subsequent to reduced fibrillin-2 cleavage
    Authors: Lauren W. Wang, Wendy E. Kutz, Timothy J. Mead, Lauren C. Beene, Shweta Singh, Michael W. Jenkins et al.
    Matrix Biology
  21. Recombinant Production of MFHR1, A Novel Synthetic Multitarget Complement Inhibitor, in Moss Bioreactors
    Authors: Oguz Top, Juliana Parsons, Lennard L. Bohlender, Stefan Michelfelder, Phillipp Kopp, Christian Busch-Steenberg et al.
    Frontiers in Plant Science
  22. A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice
    Authors: Asher J. Williams, Katherine F. Warfel, Primit Desai, Jie Li, Jen-Jie Lee, Derek A. Wong et al.
    Frontiers in Molecular Biosciences
  23. DNA methylation dynamics during B cell maturation underlie a continuum of disease phenotypes in chronic lymphocytic leukemia
    Authors: Christopher C Oakes, Marc Seifert, Yassen Assenov, Lei Gu, Martina Przekopowitz, Amy S Ruppert et al.
    Nature Genetics
  24. Bacterial muropeptides promote OXPHOS and suppress mitochondrial stress in mammals
    Authors: Tian, D;Cui, M;Han, M;
    Cell reports
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  25. Reprogramming human B cells with custom heavy chain antibodies
    Authors: Rogers, GL;Huang, C;Mathur, A;Huang, X;Chen, HY;Stanten, K;Morales, H;Chang, CH;Kezirian, EJ;Cannon, PM;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
  26. Improved yield of recombinant human IFN-alpha2b from mammalian cells using heterologous signal peptide approach
    Authors: C Wilkinson, J Kyle, M Irimpen, S Stuart, S Mohandass, A Sheperd, KJ Smith, MJ Mullin
    Protein Expression and Purification, 2022-06-02;198(0):106125.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Crystal structures of pertussis toxin with NAD+ and analogs provide structural insights into the mechanism of its cytosolic ADP-ribosylation activity
    Authors: M Sakari, MT Tran, J Rossjohn, AT Pulliainen, T Beddoe, DR Littler
    The Journal of Biological Chemistry, 2022-04-01;298(5):101892.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  28. Structural dynamics of receptor recognition and pH-induced dissociation of full-length Clostridioides difficile Toxin B
    Authors: M Jiang, J Shin, R Simeon, JY Chang, R Meng, Y Wang, O Shinde, P Li, Z Chen, J Zhang
    PloS Biology, 2022-03-24;20(3):e3001589.
    Species: N/A
    Sample Types: Complex Sample Type
    Applications: ELISA (detection)
  29. ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation
    Authors: F Okumura, N Oki, Y Fujiki, R Ikuta, K Osaki, S Hamada, K Nakatsukas, N Hisamoto, T Hara, T Kamura
    Scientific Reports, 2021-10-22;11(1):20880.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  30. Evaluation of a genus-specific rGroEL1-524 IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand
    Authors: S Maneewatch, G Doungchawe, T Kalambahet, V Luvira, N Soonthornw, P Vattanatha, U Chaisri, P Adisakwatt
    Scientific Reports, 2021-10-05;11(1):19785.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  31. Optimized expression and purification of adipose triglyceride lipase improved hydrolysis and transacylation catalytic activities in vitro
    Authors: N Kulminskay, C Radler, R Viertlmayr, C Heier, P Hofer, M Colaço-Gas, RJ Owens, R Zimmermann, R Schreiber, R Zechner, M Oberer
    The Journal of Biological Chemistry, 2021-09-17;0(0):101206.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain
    Authors: E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim
    Nature Communications, 2021-04-06;12(1):2061.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Co-IP, ICC, Immunoprecipitation, Western Blot
  33. Structure and noncanonical Cdk8 activation mechanism within an Argonaute-containing Mediator kinase module
    Authors: YC Li, TC Chao, HJ Kim, T Cholko, SF Chen, G Li, L Snyder, K Nakanishi, CE Chang, K Murakami, BA Garcia, TG Boyer, KL Tsai
    Science Advances, 2021-01-15;7(3):.
    Species: Yeast
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Forward genetics identifies a novel sleep mutant with sleep state inertia and REM sleep deficits
    Authors: GT Banks, MCC Guillaumin, I Heise, P Lau, M Yin, N Bourbia, C Aguilar, MR Bowl, C Esapa, LA Brown, S Hasan, E Tagliatti, E Nicholson, RS Bains, S Wells, VV Vyazovskiy, K Volynski, SN Peirson, PM Nolan
    Sci Adv, 2020-08-12;6(33):eabb3567.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  35. New insights into the genetic basis of premature ovarian insufficiency: Novel causative variants and candidate genes revealed by genomic sequencing
    Authors: S Jaillard, K Bell, L Akloul, K Walton, K McElreavy, WA Stocker, M Beaumont, C Harrisson, T Jääskeläin, JJ Palvimo, G Robevska, E Launay, AP Satié, N Listyasari, C Bendavid, R Sreenivasa, S Duros, J van den Be, C Henry, M Domin-Bern, L Cornevin, N Dejucq-Rai, MA Belaud-Rot, S Odent, KL Ayers, C Ravel, EJ Tucker, AH Sinclair
    Maturitas, 2020-06-20;141(0):9-19.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity
    Authors: S Ols, L Yang, EA Thompson, P Pushparaj, K Tran, F Liang, A Lin, B Eriksson, GB Karlsson H, RT Wyatt, K Loré
    Cell Rep, 2020-03-24;30(12):3964-3971.e7.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  37. Enolase and dipeptidyl peptidase IV protein sub-unit vaccines are not protective against a lethal Streptococcus suis serotype 2 challenge in a mouse model of infection
    Authors: A Dumesnil, L Martelet, D Grenier, JP Auger, J Harel, E Nadeau, M Gottschalk
    BMC Vet. Res., 2019-12-10;15(1):448.
    Species: Bacteria - Streptococcus suis
    Sample Types: Recombinant Protein
    Applications: Western Blot
  38. The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation
    Authors: MER Maitland, G Onea, CA Chiasson, X Wang, J Ma, SE Moor, KR Barber, GA Lajoie, GS Shaw, C Schild-Pou
    Sci Rep, 2019-07-08;9(1):9864.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  39. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019-06-11;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Cell Culture Supernates, Protein
    Applications: ELISA Development, Epitope Mapping
  40. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors
    Authors: M Yeste-Vela, T Guo, X Mao, E Stankiewic, G Scandura, H Li, CS Wang, S Kudahetti, T Oliver, D Berney, J Shamash, YJ Lu
    Am J Cancer Res, 2019-05-01;9(5):855-871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Knockdown
  41. The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization
    Authors: L Lei, YR Yang, K Tran, Y Wang, CI Chiang, G Ozorowski, Y Xiao, AB Ward, RT Wyatt, Y Li
    Cell Rep, 2019-04-09;27(2):586-598.e6.
    Species: Guinea Pig
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  42. Thrombin cleaves and activates the protease-activated receptor 2 dependent on thrombomodulin co-receptor availability
    Authors: DM Heuberger, AG Franchini, J Madon, RA Schuepbach
    Thromb. Res., 2019-02-28;177(0):91-101.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development (Capture)
  43. EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation
    Authors: L Herviou, M Jourdan, AM Martinez, G Cavalli, J Moreaux
    Leukemia, 2019-02-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  44. Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab
    Authors: SM Chin, CR Kimberlin, Z Roe-Zurz, P Zhang, A Xu, S Liao-Chan, D Sen, AR Nager, NS Oakdale, C Brown, F Wang, Y Yang, K Lindquist, YA Yeung, S Salek-Arda, J Chaparro-R
    Nat Commun, 2018-11-08;9(1):4679.
    Applications: ELISA (Capture)
  45. Reciprocal negative regulation between the tumor suppressor protein p53 and B cell CLL/lymphoma 6 (BCL6) via control of caspase-1 expression
    Authors: MK Kim, JY Song, DI Koh, JY Kim, M Hatano, BN Jeon, MY Kim, SY Cho, KS Kim, MW Hur
    J. Biol. Chem., 2018-11-08;0(0):.
    Applications: Western Blot
  46. C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
    Authors: S Iyer, V Subramania, KR Acharya
    PeerJ, 2018-10-17;6(0):e5815.
    Species: Insect
    Sample Types: Virus
    Applications: Western Blot
  47. rA1M-035, a physicochemically improved human recombinant ?1-microglobulin, has therapeutic effects in rhabdomyolysis-induced acute kidney injury
    Authors: B Åkerström, L Rosenlöf, A Hägerwall, S Rutardotti, J Ahlstedt, ME Johansson, L Erlandsson, M Allhorn, MG Gram
    Antioxid. Redox Signal., 2018-04-11;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  48. Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies
    Authors: F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada
    Cancer Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  49. Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein
    Authors: JH Urban, MA Moosmeier, T Aumüller, M Thein, T Bosma, R Rink, K Groth, M Zulley, K Siegers, K Tissot, GN Moll, J Prassler
    Nat Commun, 2017-11-15;8(1):1500.
    Applications: Bioassay
  50. Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Authors: D Wensel, Y Sun, Z Li, S Zhang, C Picarillo, T McDonagh, D Fabrizio, M Cockett, M Krystal, J Davis
    Antimicrob. Agents Chemother., 2017-07-25;0(0):.
    Applications: Binding Assay
  51. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
    Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
    Immunity, 2017-05-16;46(5):804-817.e7.
    Species: Primate
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  52. Hypoxia favors the generation of human plasma cells
    Authors: M Schoenhals, M Jourdan, A Bruyer, A Kassambara, B Klein, J Moreaux
    Cell Cycle, 2017-05-02;0(0):1-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  53. Structural basis of homo- and heterotrimerization of collagen I
    Authors: U Sharma, L Carrique, S Vadon-Le G, N Mariano, RN Georges, F Delolme, P Koivunen, J Myllyharju, C Moali, N Aghajari, DJ Hulmes
    Nat Commun, 2017-03-10;8(0):14671.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  54. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
    Sci Rep, 2016-11-18;6(0):36862.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cross-linking
  55. Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
    Authors: Alexey A Lugovskoy
    MAbs, 2016-11-17;0(0):0.
    Applications: Flow Cytometry
  56. Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies
    PLoS Pathog, 2016-08-03;12(8):e1005767.
    Species: Guinea Pig
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  57. A Novel Bispecific Antibody Targeting EGFR and cMet that is Effective Against EGFR Inhibitor- Resistant Lung Tumors
    Cancer Res, 2016-05-23;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Functional Assay
  58. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins
    J Immunol, 2016-05-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
    Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
    J Biol Chem, 2016-04-01;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  60. Heparan Sulfate Proteoglycans Are Important for Islet Amyloid Formation and Islet Amyloid Polypeptide-induced Apoptosis.
    Authors: Oskarsson M, Singh K, Wang J, Vlodavsky I, Li J, Westermark G
    J Biol Chem, 2015-04-28;290(24):15121-32.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Neutralization
  61. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein.
    Authors: Konitsiotis A, Jovanovic B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate E, Magee A
    J Biol Chem, 2014-12-12;290(6):3293-307.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  62. Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
    MAbs, 2014-07-01;6(4):1059-68.
  63. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
    Authors: Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M
    J Exp Med, 2014-06-23;211(7):1433-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cross-linking
  64. Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development.
    Authors: Kondo, Shunya, Bottos, Alessia, Allegood, Jeremy C, Masson, Regis, Maurer, Francisc, Genoud, Christel, Kaeser, Patrick, Huwiler, Andrea, Murakami, Masato, Spiegel, Sarah, Hynes, Nancy E
    PLoS ONE, 2014-04-08;9(4):e94114.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation.
    Authors: Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X
    J Biol Chem, 2014-03-13;289(16):11522-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  66. VB-201, an oxidized phospholipid small molecule, inhibits CD14- and Toll-like receptor-2-dependent innate cell activation and constrains atherosclerosis.
    Authors: Mendel I, Feige E, Yacov N, Salem Y, Levi I, Propheta-Meiran O, Shoham A, Ishai E, George J, Harats D, Breitbart E
    Clin Exp Immunol, 2014-01-01;175(1):126-37.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  67. Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules.
    Authors: Bai X, Bowen J, Knox T, Zhou K, Pendziwiat M, Kuhlenbaumer G, Sindelar C, Spiliotis E
    J Cell Biol, 2013-12-23;203(6):895-905.
    Species: Bovine
    Sample Types: Recombinant Protein
    Applications: Western Blot
  68. Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells.
    Authors: Matsui T, Higashimoto Y, Taira J, Yamagishi S
    Biochem Biophys Res Commun, 2013-10-23;441(2):405-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  69. Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.
    Authors: Kyathanahalli, Chandras, Marks, Jason, Nye, Kennedy, Lao, Belinda, Albrecht, Eugene D, Aberdeen, Graham W, Nathanielsz, Peter W, Jeyasuria, Panchara, Condon, Jennifer
    Endocrinology, 2013-10-18;154(12):4873-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cross-linking
  70. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors.
    Authors: Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M
    J Immunol, 2013-04-24;190(11):5739-46.
    Species: Mouse
    Sample Types: Recombinant Proteins
    Applications: Surface Plasmon Resonance (SPR
  71. Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG.
    Authors: Rose, Rebecca, van Berkel, Patrick, van den Bremer, Ewald T, Labrijn, Aran F, Vink, Tom, Schuurman, Janine, Heck, Albert J, Parren, Paul W H
    MAbs, 2013-02-13;5(2):219-28.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  72. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012-05-02;7(5):e35639.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  73. A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells.
    Authors: Simhadri VL, Hansen HP, Simhadri VR, Reiners KS, Bessler M, Engert A, von Strandmann EP
    Biol. Chem., 2012-01-01;393(1):101-6.
    Applications: Cross-linking
  74. Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
    Authors: Stephenson-Famy A, Marks J, Suresh A
    Mol. Endocrinol., 2011-12-22;26(2):320-30.
    Applications: Cross-linking
  75. C17 prevents inflammatory arthritis and associated joint destruction in mice.
    Authors: Chao C, Joyce-Shaikh B, Grein J
    PLoS ONE, 2011-07-25;6(7):e22256.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  76. Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion.
    Authors: Wu ZL, Ethen C, Hickey GE, Jiang W
    Biochem. Biophys. Res. Commun., 2009-01-06;379(3):749-53.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Western Blot
  77. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008-07-02;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  78. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008-03-13;283(20):13874-88.
    Applications: Affinity Purification
  79. The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
    Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
    J. Immunol., 2007-03-15;178(6):3379-89.
    Applications: Cross-linking
  80. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
    Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
    Int. J. Cancer, 2006-07-15;119(2):349-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  81. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
    Applications: Western Blot
  82. Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors.
    Authors: Lee FH, Haskell C, Charo IF, Boettiger D
    Biochemistry, 2004-06-08;43(22):7179-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  83. One size does not fit all: navigating the multi-dimensional space to optimize T-cell engaging protein therapeutics
    Authors: Wei Chen, Fan Yang, Carole Wang, Jatin Narula, Edward Pascua, Irene Ni et al.
    mAbs
  84. A synthetic protein as efficient multitarget regulator against complement over-activation
    Authors: Natalia Ruiz-Molina, Juliana Parsons, Madeleine Müller, Sebastian N. W. Hoernstein, Lennard L. Bohlender, Steffen Pumple et al.
    Communications Biology
  85. Dual dependence on BCL2 and MCL1 in T-cell prolymphocytic leukemia
    Authors: Victoria M. Smith, Oliver Lomas, Donna Constantine, Lianne Palmer, Anna H. Schuh, David Bruce et al.
    Blood Advances
  86. A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment
    Authors: Ladaigue S, Lefranc AC, Balde K et al.
    iScience
  87. A Combinatory Antibody–Antigen Microarray Assay for High-Content Screening of Single-Chain Fragment Variable Clones from Recombinant Libraries
    Authors: Nina Persson, Bo Jansson, Nicolai Stuhr-Hansen, András Kovács, Charlotte Welinder, Lena Danielsson et al.
    PLOS ONE
  88. The SARS-CoV-2 RNA polymerase is a viral RNA capping enzyme
    Authors: Alexander P Walker, Haitian Fan, Jeremy R Keown, Michael L Knight, Jonathan M Grimes, Ervin Fodor
    Nucleic Acids Research
  89. Differential regulation of fibroblast growth factor receptor 1 trafficking and function by extracellular galectins
    Authors: Marika Kucińska, Natalia Porębska, Agata Lampart, Marta Latko, Agata Knapik, Małgorzata Zakrzewska et al.
    Cell Communication and Signaling
  90. Crystal structures of angiotensin-converting enzyme from Anopheles gambiae in its native form and with a bound inhibitor
    Authors: John S. Cashman, Gyles E. Cozier, Charlotte Harrison, R. Elwyn Isaac, K. Ravi Acharya
    Biochemical Journal
  91. Grabody B, an IGF1 receptor-based shuttle, mediates efficient delivery of biologics across the blood-brain barrier
    Authors: Jung-Won Shin, Sungwon An, Dongin Kim, Hyunjoo Kim, Jinhyung Ahn, Jaehyun Eom et al.
    Cell Reports Methods
  92. Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability
    Authors: Viktoriya Dubrovskaya, Karen Tran, Gabriel Ozorowski, Javier Guenaga, Richard Wilson, Shridhar Bale et al.
    Immunity
  93. Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2
    Authors: Fumihiko Okumura, Akiko Joo-Okumura, Keisuke Obara, Alexander Petersen, Akihiko Nishikimi, Yoshinori Fukui et al.
    Molecular Biology of the Cell
  94. A Cancer Cell-Intrinsic GOT2-PPAR? Axis Suppresses Antitumor Immunity
    Authors: Abrego J, Sanford-Crane H, Oon C et al.
    Cancer discovery
  95. Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis
    Authors: Hiromi Imamura, Shuichiro Sakamoto, Tomoki Yoshida, Yusuke Matsui, Silvia Penuela, Dale W Laird et al.
    eLife
  96. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation.
    Authors: Uematsu K, Okumura F, Tonogai S et al.
    J. Cell Biol.
  97. Curcumin partly prevents ISG15 activation via ubiquitin-activating enzyme E1-like protein and decreases ISGylation
    Authors: Nodoka Oki, Shino Yamada, Tamaki Tanaka, Hiromi Fukui, Shigetsugu Hatakeyama, Fumihiko Okumura
    Biochemical and Biophysical Research Communications
  98. The Timing and Location of Glial Cell Line-Derived Neurotrophic Factor Expression Determine Enteric Nervous System Structure and Function
    Authors: Hongtao Wang, Inna Hughes, William Planer, Alexander Parsadanian, John R. Grider, Bhupinder P. S. Vohra et al.
    The Journal of Neuroscience

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

  2. Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?

    • MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide.  It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions.  If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.

  3. What is the difference between His Tag Antibodies, Catalog #s MAB050 and MAB050R?

    • The primary difference is how the antibodies are produced. Catalog # MAB050R is made using recombinant DNA technology while Catalog # MAB050 is produced from a hybridoma.

View all Antibody FAQs
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Average Rating: 4.8 (Based on 14 Reviews)

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His Tag Antibody
By Veronica Smith on 12/01/2023
Application: WB Sample Tested: Purified protein Species: E. coli

Used in conjunction with Licor Anti-Mouse secondary, imaged with Typhoon imager

Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 08/31/2023
Application: Flow Sample Tested: Recombinant protein Species: Mouse
Flow Cytometry His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 04/06/2022
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 10/29/2021
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 08/06/2021
Application: WB Sample Tested: Recombinant protein Species: Mouse
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 05/14/2019
Application: ELISA Sample Tested: CTLL-2 mouse cytotoxic T cell line,HT-2 mouse T cell line Species: Mouse
His Tag Antibody
By Jenna Nguyen on 05/07/2019
Application: Flow Sample Tested: 7TD1 mouse hybridoma cell line Species: Mouse
His Tag Antibody
By Jenna Nguyen on 01/11/2019
Application: Cell Depl Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse
His Tag Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

293T cells were transfected with pCDNA 3.1-Control or His-RIP1 for 48h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human His Tag Antibody (MAB050). A specific band was detected for His-RIP1 at approximately 75 kDa. This experiment was conducted under reducing conditions

Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 06/20/2018
Application: Simple Western Sample Tested: Purified protein Species: Human
Simple Western His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 07/15/2017
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Mouse
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 06/28/2016
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

cell lysates transformed

His Tag Antibody
By Rachel Jacobson on 06/28/2016
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 10/26/2015
Application: ELISA Sample Tested: Human recombinant test antibody Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + BSA
Dilution: used at 2ug/mL