HEPES
Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Purity: ≥99.5%
Biological Activity
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.For more information about how HEPES may be used, see our protocol:
3D Culture of Lung Alveolar CellsTechnical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Hydrogen ion buffers for biological research.
Good et al.
Biochemistry, 1966;5:467 -
Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
Medzon and Gedies
Canadian J.Microbiol., 1971;17:651
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Citation for HEPES
The citations listed below are publications that use Tocris products. Selected citations for HEPES include:
1 Citation: Showing 1 - 1
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Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists.
Authors: Lerner Et al.
J Neurosci 2010;30:2160
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Used at 20 mmol/L in DMEM buffer for primary cell culture
Additive in cell culture media
