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Goat Anti-Mouse IgM PE-conjugated Antibody

Catalog #: F0116
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0116
Detection of Mouse IgM Primary Antibody by Flow Cytometry
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Citations (1)
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Goat Anti-Mouse IgM PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgM
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, wherethe primary antibody does not have a fluorescent reporter, is of mouse origin, and is of the IgM class. Therecommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgM Primary Antibody by Flow Cytometry View Larger

Detection of Mouse IgM Primary Antibody by Flow Cytometry HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13882, filled histogram) or isotype control antibody mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116) at 10 µL/106cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgM

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin M
Alternate Names
AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; IgM; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH

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Citation for Goat Anti-Mouse IgM PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro*
    Authors: Letícia Martins SANTOS, Patricia e Silva CARDOSO, Elisa Abreu DINIZ, Juliana Garuba RAHHAL, Carla Renata SIPERT
    Journal of Applied Oral Science

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