Feline SCF/c-kit Ligand Biotinylated Antibody

Catalog #: BAF2268 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2268

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Feline SCF/c-kit Ligand Biotinylated Antibody Summary

Species Reactivity

Feline

Specificity

Detects feline SCF/c‑kit Ligand in Western blots. In Western blots, approximately 60% cross-reactivity with recombinant mouse SCF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant feline SCF/c‑kit Ligand
Lys26-Ala190
Accession # P79169

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Feline SCF/c-kit Ligand (Catalog # 2268-SC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

Feline SCF (stem cell factor; also known as c-kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1‑4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are two intrachain disulfide bonds and four alpha -helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28‑40 kDa in size and reflects both N- and O-linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide-linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of feline SCF has been identified (5). It too, is membrane bound and contains 246 aa residues. It will not give rise to a soluble form, since alternate splicing removes the proteolytic cleavage site used in the long form. The ratio of long form to short form varies tissue to tissue (1). Soluble feline SCF shares 93%, 93%, 90%, 87%, and 78% aa sequence identity with porcine, canine, bovine, human and mouse SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).

References

Broudy, V.C. (1997) Blood 90:1345.
Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
Kapur, R. et al. (1998) Blood 91:879.

Long Name

Stem Cell Factor

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Alternate Names

c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor