ExCellerate™ iPSC Expansion Medium, Animal-Free, GMP

Human iPSC expanded in CCM036 and CCM036-GMP show similar growth kinetics. Catalog # CCM036 (RUO version) and Catalog # CCM036-GMP have the same formulation and are manufactured with the same high quality animal-free components. No significant difference in growth kinetics is seen when culturing iPSC in either CCM036 or CCM036-GMP.

Using ExCellerate iPSC Media better supports low density iPSC culture compared to other media suppliers. Human iPSCs were plated at low density (3000 cells per well in a 6 well plate) and cultured until the cells reached approximately 70% confluence for 3 passages. Cells were harvested using Accutase and live cells were counted. All groups were then normalized to CCM036-GMP. Competitors showed slower and highly variable cell growth (Plot A) as well as lower viability (Plot B) compared to CCM036-GMP.

ExCellerate™ iPSC Expansion Medium maintain expression of stemness markers over long-term cultures (at least 24-passages). Human iPSC expanded in CCM036-GMP express key stemness markers, Oct 3/4 and Nanog. SSEA-1 used as negative control.

Stable genotype over long-term culture. Human iPSCs cultured in CCM036-GMP show characteristics healthy colony morphology including clearly defined edges, compact and tightly packed cells, along with normal karyotype after 26 passages.

Representative images of iPSC expansion and differentiation.
Image A shows iPSCs expanded in culture using ExCellerate iPSC Expansion Medium (Catalog # CCM036-GMP).
Image B shows the differentiation into PDX-1 expressing pancreatic progenitors (AF2419).
Image C shows the presence of large population of C-peptide (MAB14171) expressing monohormonal beta cells.
Image D shows the co-expression of PDX-1 and C-peptide in the population.

iPSC grown in CCM036-GMP retain their pluripotency. After expansion in CCM036-GMP, cells were differentiated to the three germ layers: endoderm (SOX17), mesoderm (Brachyury), and ectoderm (OTX2).