Rabbit IgG NorthernLights™ NL637-conjugated Antibody

NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL005
Detection of Rabbit IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (4)
FAQs
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Rabbit IgG NorthernLights™ NL637-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL637 (Excitation= 637 nm, Emission= 658 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Rabbit IgG Primary Antibody by Immunocytochemistry. Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperarture. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG NorthernLights™ NL637-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The IL-17A-neutrophil axis promotes epithelial cell IL-33 production during nematode lung migration
    Authors: Ajendra, J;Papotto, PH;Parkinson, JE;Dodd, RJ;Bombeiro, AL;Pearson, S;Chan, BHK;Ribot, JC;McSorley, HJ;Sutherland, TE;Allen, JE;
    Mucosal immunology
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. IL-13 deficiency exacerbates lung damage and impairs epithelial-derived type 2 molecules during nematode infection
    Authors: Alistair L Chenery, Silvia Rosini, James E Parkinson, Jesuthas Ajendra, Jeremy A Herrera, Craig Lawless et al.
    Life Science Alliance
  3. A micro-sterile inflammation array as an adjuvant for influenza vaccines.
    Authors: Wang J, Shah D, Chen X, Anderson R, Wu M
    Nat Commun, 2014-07-18;5(0):4447.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. mTORC1 and mTORC2 play different roles in the functional survival of transplanted adipose-derived stromal cells in hind limb ischemic mice via regulating inflammation in vivo.
    Authors: Fan W, Cheng K, Qin X, Narsinh K, Wang S, Hu S, Wang Y, Chen Y, Wu J, Xiong L, Cao F
    Stem Cells, 2013-01-01;31(1):203-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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