DermaTACs In Situ Apoptosis Detection Kit Summary
Provides researchers with an effective method for measuring apoptosis in skin samples using DNA end-labeling using terminal deoxynucleotidyl transferase (TdT), modified nucleotides (BrdU).Key Benefits
• Performance tested on dermal tissues.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Helps resolve unique problems encountered when detecting apoptosis in dermal tissue.
Why Use the DermaTACS In Situ Apoptosis Detection Kit?
The DermaTACS Kit was developed to provide researchers with an effective method for measuring apoptosis in skin samples. The kit was based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT), modified nucleotides (BrdU). Detection of incorporated molecules is achieved using a biotinylated BrdU antibody and chromogenic substrate with a Streptavidin horseradish peroxidase detection system.
The extracellular scaffolding in skin tissue can make it problematic to permeabilize and high background is a common problem with skin samples. The DermaTACS Kit includes two permeabilization reagents as options for permeabilization along with a detailed protocol with hints and tips for optimal labeling of skin samples based on empirical testing. Low background is achieved using a combination of optimized reagents and visualization methods. Also included is TACS-Nuclease to generate a positive control from your own sample. In conjunction with the excellent contrast between the TACS Blue Label and the Red Counterstain C, the positive control ensures easy data interpretation.
Kit Contents
• Streptavidin-HRP
• TACS-Blue Label
• TACS-Nuclease
• TACS-Nuclease Buffer
• Red Counterstain C
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• B-dNTP Mix
• anti-BrdU antibody
• Strep-Diluent
• Cytonin
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for DermaTACs In Situ Apoptosis Detection Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer Hyperacetylation
Authors: MR Wilson, JJ Reske, J Holladay, S Neupane, J Ngo, N Cuthrell, M Wegener, M Rhodes, M Adams, R Sheridan, G Hostetter, FT Alotaibi, PJ Yong, MS Anglesio, BA Lessey, RE Leach, JM Teixeira, SA Missmer, AT Fazleabas, RL Chandler
Cell Rep, 2020-11-10;33(6):108366. 2020-11-10
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Ethanol consumption synergistically increases ultraviolet radiation induced skin damage and immune dysfunction
Authors: RM Brand, JM Stottlemye, MC Paglia, CD Carey, LD Falo
J Dermatol Sci, 2020-11-05;0(0):. 2020-11-05
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Tumor-Preferential Induction of Immune Responses and Epidermal Cell Death in Actinic Keratoses by Ingenol Mebutate
PLoS ONE, 2016-09-09;11(9):e0160096. 2016-09-09
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Targeted deletion of RasGRP1 impairs skin tumorigenesis.
Authors: Sharma A, Fonseca L, Rajani C, Yanagida J, Endo Y, Cline J, Stone J, Ji J, Ramos J, Lorenzo P
Carcinogenesis, 2014-01-24;35(5):1084-91. 2014-01-24
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Chemotherapy targets the hair-follicle vascular network but not the stem cells.
Authors: Amoh Y, Li L, Katsuoka K, Hoffman RM
J. Invest. Dermatol., 2006-07-13;127(1):11-5. 2006-07-13
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This kit was ordered from Trevigen (order number 64163) and used a few years ago. It was the positive control using Proteinase K and TACS-Nuclease in mouse skin.
