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Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

Catalog #: 3434-010-02
13 Citations Datasheet / COA / SDS
Basement membrane extract specifically qualified to support feeder-free stem cell culture for embryonic and pluripotent stem cells
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Catalog # Availability Size / Price Qty
3434-001-02
3434-005-02
3434-010-02
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Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs).
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Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract Summary

Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.
 

Key Benefits

• Qualified to support the culture of pluripotent stem cell
• Supports human iPSC and ESC expansion and differentiation
• Reduced growth factor formulation provides a more defined culture system
• Quality controlled for performance consistency

Why Use Cultrex Stem Cell Qualified RGF BME?

Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex Stem Cell Qualified RGF BME has been shown to provide an effective surface for feeder-free stem cell attachment and maintenance of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is useful for promoting the expansion of pluripotent stem cells or for the study of stem cell differentiation.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Promotes the human induced pluripotent stem cell attachment.

Effectively maintains human iPSCs in a pluripotent state in a feeder-free stem cell culture.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Scientific Data

Flow Cytometry Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs). View Larger

Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs). Human iPSCs were grown for a minimum of 2 passages on either Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02) or a leading competitor's matrix, prior to creating embryoid bodies for hematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME, showed a greater efficiency to develop into hematopoietic stem cells (CD34+, CD45+). Data courtesy of the Verneris Laboratory at the University of Colorado.

Stem Cell Colonies of Human iPSCs Grown on Cultrex Stem Cell Qualified RGF BME. View Larger

Colonies of iPSCs Grown on Cultrex Stem Cell Qualified RGF BME. Human erythroblasts, reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4X magnification. Data courtesy of the Harvard Stem Cell Core.

Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Generation of iPSCs from identical twin, one affected by LHON and one unaffected, both carrying a combination of two mitochondrial variants: m.14484 T>C and m.10680G>A
    Authors: Peron, C;Cavaliere, A;Fasano, C;Iannielli, A;Spagnolo, M;Legati, A;Nicol Colombo, M;Rizzo, A;Sciacca, FL;Carelli, V;Broccoli, V;Lamperti, C;Tiranti, V;
    Stem cell research  2024-03-24
  2. A pharmacological toolkit for human microglia identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer's disease
    Authors: Haage, V;Tuddenham, JF;Comandante-Lou, N;Bautista, A;Monzel, A;Chiu, R;Fujita, M;Garcia, FG;Bhattarai, P;Patel, R;Buonfiglioli, A;Idiarte, J;Herman, M;Rinderspacher, A;Mela, A;Zhao, W;Argenziano, MG;Furnari, JL;Banu, MA;Landry, DW;Bruce, JN;Canoll, P;Zhang, Y;Nuriel, T;Kizil, C;Sproul, AA;de Witte, LD;Sims, PA;Menon, V;Picard, M;De Jager, PL;
    bioRxiv : the preprint server for biology  2024-02-06
  3. Co-fractionation-mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain
    Authors: Zilocchi, M;Rahmatbakhsh, M;Moutaoufik, MT;Broderick, K;Gagarinova, A;Jessulat, M;Phanse, S;Aoki, H;Aly, KA;Babu, M;
    Nature protocols  2023-11-20
  4. Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex
    Authors: Winden, KD;Pham, TT;Teaney, NA;Ruiz, J;Chen, R;Chen, C;Sahin, M;
    Cell reports  2023-07-25
  5. Parkin regulates amino acid homeostasis at mitochondria-lysosome (M/L) contact sites in Parkinson's disease
    Authors: Peng, W;Schröder, LF;Song, P;Wong, YC;Krainc, D;
    Science advances  2023-07-21
  6. Genetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types
    Authors: Maggiore, JC;LeGraw, R;Przepiorski, A;Velazquez, J;Chaney, C;Streeter, E;Silva-Barbosa, A;Franks, J;Hislop, J;Hill, A;Wu, H;Pfister, K;Howden, SE;Watkins, SC;Little, M;Humphreys, BD;Watson, A;Stolz, DB;Kiani, S;Davidson, AJ;Carroll, TJ;Cleaver, O;Sims-Lucas, S;Ebrahimkhani, MR;Hukriede, NA;
    bioRxiv : the preprint server for biology  2023-05-30
  7. Modeling gene � environment interactions in PTSD using human neurons reveals diagnosis-specific glucocorticoid-induced gene expression
    Authors: C Seah, MS Breen, T Rusielewic, HN Bader, C Xu, CJ Hunter, B McCarthy, PJM Deans, M Chattopadh, J Goldberg, F Desarnaud, I Makotkine, JD Flory, LM Bierer, M Staniskyte, NYSCF Glob, SA Noggle, LM Huckins, D Paull, KJ Brennand, R Yehuda
    Nature Neuroscience, 2022-10-20;25(11):1434-1445.  2022-10-20
  8. Dominant role of DNA methylation over H3K9me3 for IAP silencing in endoderm
    Authors: Z Wang, R Fan, A Russo, FM Cernilogar, A Nuber, S Schirge, I Shcherbako, I Dzhilyanov, E Ugur, T Anton, L Richter, H Leonhardt, H Lickert, G Schotta
    Nature Communications, 2022-09-19;13(1):5447.  2022-09-19
  9. Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas
    Authors: MP Pappas, N Xie, JS Penaloza, SSK Chan
    Cells, 2022-05-09;11(9):.  2022-05-09
  10. NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency
    Authors: KJ Choi, MD Quan, C Qi, JH Lee, PS Tsoi, M Zahabiyon, A Bajic, L Hu, BVV Prasad, SJ Liao, W Li, ACM Ferreon, JC Ferreon
    Nature Cell Biology, 2022-04-28;0(0):.  2022-04-28
  11. Generation of two human iPSC lines, FINCBi002-A and FINCBi003-A, carrying heteroplasmic macrodeletion of mitochondrial DNA causing Pearson's syndrome
    Authors: C Peron, R Mauceri, A Iannielli, A Cavaliere, A Legati, A Rizzo, FL Sciacca, V Broccoli, V Tiranti
    Stem Cell Research, 2021-01-04;50(0):102151.  2021-01-04
  12. Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leber�s Hereditary optic Neuropathy (LHON)
    Authors: C Peron, R Mauceri, T Cabassi, A Segnali, A Maresca, A Iannielli, A Rizzo, FL Sciacca, V Broccoli, V Carelli, V Tiranti
    Stem Cell Res, 2020-08-03;48(0):101939.  2020-08-03
  13. Doublecortin like kinase 1 regulates alpha-Synuclein levels and toxicity
    Authors: GE Vázquez-Vé, KA Gonzales, JP Revelli, CJ Adamski, F Alavi Nain, A Baji?, E Craigen, R Richman, SM Heman-Acka, MJA Wood, MWC Rousseaux, HY Zoghbi
    J. Neurosci., 2019-11-20;0(0):.  2019-11-20

FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

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