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Cultrex Basement Membrane Extract, PathClear

Catalog #: 3432-010-01
50 Citations 9 Reviews Datasheet / COA / SDS
Basement Membrane Extract for use in multiple culture applications
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3432-001-01
3432-005-01
3432-010-01
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Cultrex Basement Membrane Extract
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Citations (50)
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Reviews (9)
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Cultrex Basement Membrane Extract, PathClear Summary

Cultrex Basement Membrane Extract (BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion.
 

Key Benefits

• Ideal for general cell culture application
• Polymerizes at 37°C to form a reconstituted basement membrane
• Quality controlled for peformance consistency

Why Use Cultrex BME?

Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Tube Formation Assay - Cultrex BME supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells in a tube formation assay.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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Citations for Cultrex Basement Membrane Extract, PathClear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

50 Citations: Showing 1 - 10
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  1. Integration of single-nucleus and exosome RNA sequencing dissected inter-cellular communication and biomarkers in pancreatic ductal adenocarcinoma
    Authors: Tang, R;Zhang, Z;Xu, J;Wang, W;Meng, Q;Liu, Y;Du, Q;Liang, C;Hua, J;Zhang, B;Yu, X;Shi, S;
    Computational and structural biotechnology journal  2024-12-01
  2. Interaction between estrogen receptor-? and PNPLA3 p.I148M variant drives fatty liver disease susceptibility in women
    Authors: Cherubini, A;Ostadreza, M;Jamialahmadi, O;Pelusi, S;Rrapaj, E;Casirati, E;Passignani, G;Norouziesfahani, M;Sinopoli, E;Baselli, G;Meda, C;Dongiovanni, P;Dondossola, D;Youngson, N;Tourna, A;Chokshi, S;Bugianesi, E;EPIDEMIC Study Investigators, ;Della Torre, S;Prati, D;Romeo, S;Valenti, L;
    Nature medicine  2023-09-25
  3. Hyaluronic acid-based dual network hydrogel with sustained release of platelet-rich plasma as a diabetic wound dressing
    Authors: Zhou, J;Liu, Y;Liu, X;Wan, J;Zuo, S;Pan, T;Liu, Y;Sun, F;Gao, M;Yu, X;Zhou, W;Xu, J;Zhou, Z;Wang, S;
    Carbohydrate polymers  2023-08-15
  4. Reconstitution of morphogen shuttling circuits
    Authors: Zhu, R;Santat, LA;Markson, JS;Nandagopal, N;Gregrowicz, J;Elowitz, MB;
    Science advances  2023-07-14
  5. Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins
    Authors: Blümke, A;Ijeoma, E;Simon, J;Wellington, R;Purwaningrum, M;Doulatov, S;Leber, E;Scatena, M;Giachelli, CM;
    Research square  2023-07-07
  6. Direct comparison of [18F]AlF-NOTA-JR11 and [18F]AlF-NOTA-octreotide for PET imaging of neuroendocrine tumors: Antagonist versus agonist
    Authors: S Ahenkorah, C Cawthorne, E Murce, CM Deroose, T Cardinaels, Y Seimbille, G Bormans, M Ooms, F Cleeren
    Nuclear medicine and biology, 2023-03-29;118(0):108338.  2023-03-29
  7. VEGFA-modified DPSCs combined with LC-YE-PLGA NGCs promote facial nerve injury repair in rats
    Authors: W Xu, X Xu, L Yao, B Xue, H Xi, X Cao, G Piao, S Lin, X Wang
    Heliyon, 2023-03-28;9(4):e14626.  2023-03-28
  8. Extracellular vesicles-transferred SBSN drives glioma aggressiveness by activating NF-kappaB via ANXA1-dependent ubiquitination of NEMO
    Authors: H Chen, X Chen, Z Zhang, W Bao, Z Gao, D Li, X Xie, P Zhou, C Yang, Z Zhou, J Pan, X Kuang, R Tang, Z Feng, L Zhou, D Zhu, J Yang, L Wang, H Huang, D Tang, J Liu, L Jiang
    Oncogene, 2022-10-31;0(0):.  2022-10-31
  9. YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma
    Authors: W Yu, C Zhang, Y Wang, X Tian, Y Miao, F Meng, L Ma, X Zhang, J Xia
    Cancer Gene Therapy, 2022-09-19;0(0):.  2022-09-19
  10. Targeted immunotherapy against distinct cancer-associated fibroblasts overcomes treatment resistance in refractory HER2+ breast tumors
    Authors: EI Rivas, J Linares, M Zwick, A Gómez-Llon, M Guiu, A Labernadie, J Badia-Rame, A Lladó, L Bardia, I Pérez-Núñe, C Martínez-C, N Tarazona, A Sallent-Ar, M Garrido, T Celià-Terr, O Burgués, RR Gomis, J Albanell, A Calon
    Nature Communications, 2022-09-09;13(1):5310.  2022-09-09
  11. Oropouche orthobunyavirus infection is mediated by the cellular host factor Lrp1
    Authors: MM Schwarz, DA Price, SS Ganaie, A Feng, N Mishra, RM Hoehl, F Fatma, SH Stubbs, SPJ Whelan, X Cui, T Egawa, DW Leung, GK Amarasingh, AL Hartman
    Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2204706119.  2022-08-08
  12. Maladaptive functional changes in alveolar fibroblasts due to perinatal hyperoxia impair epithelial differentiation
    Authors: MR Riccetti, MG Ushakumary, M Waltamath, J Green, J Snowball, SE Dautel, M Endale, B Lami, J Woods, SK Ahlfeld, AT Perl
    JCI Insight, 2022-03-08;0(0):.  2022-03-08
  13. Development of a Gene-Activated Scaffold Incorporating Multifunctional Cell-Penetrating Peptides for pSDF-1alpha Delivery for Enhanced Angiogenesis in Tissue Engineering Applications
    Authors: RN Power, BL Cavanagh, JE Dixon, CM Curtin, FJ O'Brien
    International Journal of Molecular Sciences, 2022-01-27;23(3):.  2022-01-27
  14. Extracellular Matrix Components Regulate Bone Sialoprotein Expression in MDA-MB-231 Breast Cancer Cells
    Authors: F Keller, R Bruch, F Clauder, M Hafner, R Rudolf
    Cells, 2021-05-24;10(6):.  2021-05-24
  15. Basement membrane proteins as a substrate for efficient Trypanosoma brucei differentiation in vitro
    Authors: F Rojas, M Cayla, KR Matthews
    PloS Neglected Tropical Diseases, 2021-04-28;15(4):e0009284.  2021-04-28
  16. Obesity alters ovarian folliculogenesis through disrupted angiogenesis from increased IL-10 production
    Authors: PK Yang, CH Chou, CC Huang, WF Wen, HF Chen, CT Shun, HN Ho, MJ Chen
    Molecular Metabolism, 2021-02-13;49(0):101189.  2021-02-13
  17. Specific and Non-Invasive Fluorescent Labelling of Extracellular Vesicles for Evaluation of Intracellular Processing by Intestinal Epithelial Cells
    Authors: MS Hansen, ISE Gadegaard, EC Arnspang, K Blans, LN Nejsum, JT Rasmussen
    Biomedicines, 2020-07-14;8(7):.  2020-07-14
  18. New generation breast cancer cell lines developed from patient-derived xenografts
    Authors: J Finlay-Sch, BM Jacobsen, D Riley, KV Paul, S Turner, A Ferreira-G, JC Harrell, P Kabos, CA Sartorius
    Breast Cancer Res., 2020-06-23;22(1):68.  2020-06-23
  19. Analysis of protein chlorination by mass spectrometry
    Authors: T Nybo, MJ Davies, A Rogowska-W
    Redox Biol, 2019-06-01;26(0):101236.  2019-06-01
  20. Mitophagy regulates mitochondrial network signaling, oxidative stress, and apoptosis during myoblast differentiation
    Authors: BL Baechler, D Bloemberg, J Quadrilate
    Autophagy, 2019-04-07;0(0):.  2019-04-07
  21. Involvement of the heparanase procoagulant domain in bleeding and wound healing.
    Authors: Crispel Y, Ghanem S, Attias J, Kogan I, Brenner B, Nadir Y
    J Thromb Haemost, 2017-06-04;15(7):1463-1472.  2017-06-04
  22. Mouse embryonic dorsal root ganglia contain pluripotent stem cells that show features similar to embryonic stem cells and induced pluripotent stem cells.
    Authors: Ogawa R, Fujita K, Ito K
    Biol Open, 2017-05-15;6(5):602-618.  2017-05-15
  23. p16 controls epithelial cell growth and suppresses carcinogenesis through mechanisms that do not require RB1 function.
    Authors: Sen M, Akeno N, Reece A, Miller A, Simpson D, Wikenheiser-Brokamp K
    Oncogenesis, 2017-04-17;6(4):e320.  2017-04-17
  24. Preclinical Antitumor Efficacy of BAY 1129980-a Novel Auristatin-Based Anti-C4.4A (LYPD3) Antibody-Drug Conjugate for the Treatment of Non-Small Cell Lung Cancer.
    Authors: Willuda J, Linden L, Lerchen H, Kopitz C, Stelte-Ludwig B, Pena C, Lange C, Golfier S, Kneip C, Carrigan P, McLean K, Schuhmacher J, von Ahsen O, Muller J, Dittmer F, Beier R, El Sheikh S, Tebbe J, Leder G, Apeler H, Jautelat R, Ziegelbauer K, Kreft B
    Mol Cancer Ther, 2017-03-14;16(5):893-904.  2017-03-14
  25. Markers of oxidative stress in umbilical cord blood from G6PD deficient African newborns.
    Authors: Stadem P, Hilgers M, Bengo D, Cusick S, Ndidde S, Slusher T, Lund T
    PLoS ONE, 2017-02-24;12(2):e0172980.  2017-02-24
  26. Dual Targeting of Mesenchymal and Amoeboid Motility Hinders Metastatic Behavior.
    Authors: Jones B, Kelley L, Loskutov Y, Marinak K, Kozyreva V, Smolkin M, Pugacheva E
    Mol Cancer Res, 2017-02-24;15(6):670-682.  2017-02-24
  27. Wnt5a Drives an Invasive Phenotype in Human Glioblastoma Stem-like Cells.
    Authors: Binda E, Visioli A, Giani F, Trivieri N, Palumbo O, Restelli S, Dezi F, Mazza T, Fusilli C, Legnani F, Carella M, Di Meco F, Duggal R, Vescovi A
    Cancer Res, 2016-12-23;77(4):996-1007.  2016-12-23
  28. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-14;0(0):.  2016-11-14
  29. Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
    Cancer Res, 2016-08-30;0(0):.  2016-08-30
  30. Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy.
    Authors: Jaykumar A, Caceres P, Sablaban I, Tannous B, Ortiz P
    Am J Physiol Renal Physiol, 2015-11-04;310(2):F183-91.  2015-11-04
  31. Dickkopf-3 regulates prostate epithelial cell acinar morphogenesis and prostate cancer cell invasion by limiting TGF-beta-dependent activation of matrix metalloproteases.
    Authors: Romero D, Al-Shareef Z, Gorrono-Etxebarria I, Atkins S, Turrell F, Chhetri J, Bengoa-Vergniory N, Zenzmaier C, Berger P, Waxman J, Kypta R
    Carcinogenesis, 2015-10-26;37(1):18-29.  2015-10-26
  32. MELK-T1, a small-molecule inhibitor of protein kinase MELK, decreases DNA-damage tolerance in proliferating cancer cells.
    Authors: Beke L, Kig C, Linders J, Boens S, Boeckx A, van Heerde E, Parade M, De Bondt A, Van den Wyngaert I, Bashir T, Ogata S, Meerpoel L, Van Eynde A, Johnson C, Beullens M, Brehmer D, Bollen M
    Biosci Rep, 2015-10-02;35(6):.  2015-10-02
  33. Cabozantinib (XL184) Inhibits Growth and Invasion of Preclinical TNBC Models.
    Authors: Sameni M, Tovar E, Essenburg C, Chalasani A, Linklater E, Borgman A, Cherba D, Anbalagan A, Winn M, Graveel C, Sloane B
    Clin Cancer Res, 2015-10-02;22(4):923-34.  2015-10-02
  34. The MEF2-HDAC axis controls proliferation of mammary epithelial cells and acini formation in vitro.
    Authors: Clocchiatti A, Di Giorgio E, Viviani G, Streuli C, Sgorbissa A, Picco R, Cutano V, Brancolini C
    J Cell Sci, 2015-09-24;128(21):3961-76.  2015-09-24
  35. PI3K/mTOR dual inhibitor VS-5584 preferentially targets cancer stem cells.
    Authors: Kolev V, Wright Q, Vidal C, Ring J, Shapiro I, Ricono J, Weaver D, Padval M, Pachter J, Xu Q
    Cancer Res, 2014-11-28;75(2):446-55.  2014-11-28
  36. The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
    Authors: Bittencourt F, Wu S, Bridges J, Miller W
    J Virol, 2014-08-06;88(20):11811-24.  2014-08-06
  37. The nuclear receptor peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) promotes oncogene-induced cellular senescence through repression of endoplasmic reticulum stress.
    Authors: Zhu B, Ferry C, Markell L, Blazanin N, Glick A, Gonzalez F, Peters J
    J Biol Chem, 2014-06-04;289(29):20102-19.  2014-06-04
  38. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
    Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
    J Virol, 2014-03-12;88(10):5706-17.  2014-03-12
  39. Inhibition of tumor growth and angiogenesis by SP-2, an anti-lectin, galactoside-binding soluble 3 binding protein (LGALS3BP) antibody.
    Authors: Traini S, Piccolo E, Tinari N, Rossi C, La Sorda R, Spinella F, Bagnato A, Lattanzio R, D'Egidio M, Di Risio A, Tomao F, Grassadonia A, Piantelli M, Natoli C, Iacobelli S
    Mol Cancer Ther, 2014-02-19;13(4):916-25.  2014-02-19
  40. The interplay between hypoxia, endothelial and melanoma cells regulates vascularization and cell motility through endothelin-1 and vascular endothelial growth factor.
    Authors: Spinella F, Caprara V, Cianfrocca R, Rosano L, Di Castro V, Garrafa E, Natali P, Bagnato A
    Carcinogenesis, 2014-01-28;35(4):840-8.  2014-01-28
  41. Suppression of ERbeta signaling via ERbeta knockout or antagonist protects against bladder cancer development.
    Authors: Hsu I, Chuang K, Slavin S, Da J, Lim W, Pang S, O'Brien J, Yeh S
    Carcinogenesis, 2013-10-22;35(3):651-61.  2013-10-22
  42. Regulation of P21-activated kinase-4 by progesterone and tumor necrosis factor-alpha in human endometrium and its increased expression in advanced-stage endometriosis.
    Authors: Kim S, Kim S, Ihm H, Oh Y, Chae H, Kim C, Kang B
    J Clin Endocrinol Metab, 2013-01-04;98(2):E238-48.  2013-01-04
  43. Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells.
    Authors: Zhang W, Kai K, Choi D, Iwamoto T, Nguyen Y, Wong H, Landis M, Ueno N, Chang J, Qin L
    Proc Natl Acad Sci U S A, 2012-10-29;109(46):18707-12.  2012-10-29
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  45. HIV-1 matrix protein p17 promotes angiogenesis via chemokine receptors CXCR1 and CXCR2.
    Authors: Caccuri F, Giagulli C, Bugatti A, Benetti A, Alessandri G, Ribatti D, Marsico S, Apostoli P, Slevin M, Rusnati M, Guzman C, Fiorentini S, Caruso A
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    Cancer Res, 2012-02-29;72(9):2440-53.  2012-02-29
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FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  4. What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?

    • Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.

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Reviews for Cultrex Basement Membrane Extract, PathClear

Average Rating: 4.9 (Based on 9 Reviews)

5 Star
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Cultrex Basement Membrane Extract, PathClear
By Anonymous on 10/16/2023
Cultrex Basement Membrane Extract, PathClear 3432-010-01
Cultrex Basement Membrane Extract, PathClear
By Anonymous on 05/25/2023
Cultrex Basement Membrane Extract, PathClear 3432-010-01
Cultrex Basement Membrane Extract, PathClear
By Anonymous on 05/15/2023
Cultrex Basement Membrane Extract, PathClear 3432-010-01
Cultrex Basement Membrane Extract, PathClear
By Anonymous on 11/06/2022
Cultrex Basement Membrane Extract, PathClear 3432-010-01
Cultrex Basement Membrane Extract, PathClear
By Anonymous on 05/23/2022

Used for 3D culture experiments. Went very well

Cultrex Basement Membrane Extract, PathClear 3432-010-01
Cultrex Basement Membrane Extract, PathClear
By Anonymous on 02/01/2022

for 3D cell culture

Cultrex Basement Membrane Extract, PathClear 3432-010-01
Cultrex PathClear Basement Membrane Extract (2 x 5 mL)
By Anonymous on 08/20/2018
Application: In vivo study
Cultrex PathClear Basement Membrane Extract
By Leslie Priddy on 04/01/2018
Application: Cell migration/motility
Cultrex PathClear Basement Membrane Extract
By Leslie Priddy on 04/01/2018
Application: Cell Proliferation