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Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Catalog #: 3532-010-02P Datasheet / COA / SDS
For Robust Organoid Cultures - Basement Membrane Extract
Catalog # Availability Size / Price Qty
3532-005-02P
3532-010-02P
Cultrex Basement Membrane Extract, Type 2 (with Phenol Red)
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Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red Summary

Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.

Why Use Cultrex BME, Type 2?

Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that has a high storage modulus and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Kit Contents

• Cultrex Basement Membrane Extract (BME), Type 2
• Phenol Red

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells.

Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C.

Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates.

Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  3. What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

    • Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME.  The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595;  Corning 24-well, Catalog #3526;  ThermoFisher 6-well,  Catalog # 140675.

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