CRANAD 2

Biological Activity

Used for: amyloid- detection in vitro, tissue and in vivo. Shown to bind to plaques in APP-PS1 transgenic mice, in vitro. Detects senile plaques in 19-month-old Tg2576 mice in vivo.

Application: Fluorescence microscopy, epifluorescence microscopy.

Properties and Photophysical Data: CRANAD 2 binds to Aβ40 aggregates (K_d = 38 nM) and elicits upon interacting with Aβ aggregates, an emission blue shift (from 805 nm to 715 nm). When unbound in PBS, excitation and emission maxima (λ) are 640 nm and 805 nm, respectively.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits.
   Ran et al.
   J.Am.Chem.Soc., 2009;131:15257
2. Non-conjugated small molecule FRET for differentiating monomers from higher molecular weight amyloid beta species.
   Ran et al.
   PLoS One., 2011;6:e19362

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