Ch 55

Biological Activity

Ch 55 is a highly potent synthetic retinoid that has high affinity for RAR-α and RAR-β receptors and low affinity for cellular retinoic acid binding protein (CRABP). Inhibits rabbit tracheal epithelial cell differentiation by inhibiting transglutaminase and increasing cholesterol sulfate (EC₅₀ values are 0.02 and 0.03 nM respectively). Induces differentiation of embryonic carcinoma F9 and melanoma S91 cells (EC₅₀ values are 0.26 and 0.5 nM respectively) and inhibits the induction of ornithine decarboxylase activity in 3T6 fibroblasts (EC₅₀ = 1 nM).

For more information about how Ch 55 may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
   X Li, D Liu, Y Ma, X Du, J Jing, L Wang, B Xie, D Sun, S Sun, X Jin, X Zhang, T Zhao, J Guan, Z Yi, W Lai, P Zheng, Z Huang, Y Chang, Z Chai, J Xu, H Deng
   Cell Stem Cell, 2017;0(0):.
2. New benzoic acid derivatives with retinoid activity: lack of direct correlation between biological activity and binding to cellular retinoic acid binding protein.
   Jetten et al.
   Cancer Res., 1987;47:3523
3. Functional studies of newly sythesized benzoic acid derivatives: identification of highly potent retinoid-like activity.
   Sato et al.
   J.Cell.Physiol., 1988;135:179
4. Expression of retinoic acid receptor genes and the ligand-binding selectivity of retinoic acid receptors (RAR's).
   Hashimoto et al.
   Biochem.Biophys.Res.Comm., 1990;166:1300

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