Cell Activation Cocktail 500X
Biological Activity
Cell Activation Cocktail 500X contains the following compounds: Monensin sodium salt (1.5 mM), Phorbol 12-myristate 13-acetate (0.0405 mM) and Ionomycin calcium salt (0.67 mM). Cocktail is provided as 1 ml of 500X concentrate in ethanol.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy
A Rodriguez-, RC Lynn, M Poussin, MA Eiva, LC Shaw, RS O'Connor, NG Minutolo, V Casado-Med, G Lopez, T Matsuyama, DJ Powell
Nature Communications, 2021;12(1):877.
Product Datasheets
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Average Rating: 5 (Based on 2 Reviews)
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This product was used to perform a TH17 differentiation assay using a flow cytometry analysis afterwards. This cocktail has made cell activation easier and my experimental preparation move along much faster.
Tumor infiltrating lymphocytes (TILs) were isolated from mouse liver tumors and then stimulated with 1X cell activation cocktail (diluted in RPMI media). After 5 hour activation, cytokine production on the TILs was measured via flow cytometry. Example picture shows CD107A expression before and after activation.
Always use it at 1X.
