Carboxymethyl Lysine Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CML-adducted Protein by Western Blot. Western blot shows 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers. PVDF membrane was probed with 1 µg/mL Carboxymethyl Lysine Monoclonal Antibody (Catalog # MAB3247) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Greater band intensity for the IgG heavy chain (IgG hc) suggests increased glycation in diabetic sera. For additional reference, carboxymethyl lysine-adducted (CML) Leptin and carboxyethyl lysine-adducted (CEL) Leptin were included. A specific band for CML-adducted Leptin was detected (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxymethyl Lysine
N epsilon -carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
Product Datasheets
Citations for Carboxymethyl Lysine Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Plasma Protein Pentosidine and Carboxymethyllysine, Biomarkers for Age-related Macular Degeneration*
Authors: Jiaqian Ni, Xianglin Yuan, Jiayin Gu, Xiuzhen Yue, Xiaorong Gu, Ram H. Nagaraj et al.
Molecular & Cellular Proteomics
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Chilean papaya (Vasconcellea pubescens A. DC.) residues as a source of bioactive compounds: Chemical composition, antioxidant capacity, and antiglycation effects
Authors: Pino-Ramos, LL;Farias, DR;Olivares-Caro, L;Mitsi, C;Mardones, C;Echeverria, J;Avila, F;Gutierrez, M;
Heliyon
Species: Plant
Sample Types: Cell Extract
Applications: Western Blot -
Advanced glycation end product (AGE) targeting antibody SIWA318H is efficacious in preclinical models for pancreatic cancer
Authors: Rossi, GR;Jensen, A;Ng, S;Yin, Z;Li, A;Misra, A;Von Hoff, DD;Gruber, L;Gruber, M;Han, H;
Scientific reports
Applications: Positive Control -
Saccharomyces cerevisiae DJ-1 paralogs maintain genome integrity through glycation repair of nucleic acids and proteins
Authors: Susarla, G;Kataria, P;Kundu, A;D'Silva, P;
eLife
Species: Yeast
Sample Types: Cell Lysates
Applications: Western Blot -
Serial Passaging of RAW 264.7 Cells Modulates Intracellular AGE Formation and Downregulates RANKL-Induced In Vitro Osteoclastogenesis
Authors: TT Lucy, ANM Mamun-Or-R, M Yagi, Y Yonei
International Journal of Molecular Sciences, 2022-02-21;23(4):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration
Authors: HH Dieguez, JS Calanni, HE Romeo, A Alaimo, MF González F, A Iaquinandi, MS Chianelli, MI Keller Sar, PH Sande, RE Rosenstein, D Dorfman
Cell Death & Disease, 2021-12-04;12(12):1128.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism
Authors: L Senatus, R López-Díez, L Egaña-Gorr, J Liu, J Hu, G Daffu, Q Li, K Rahman, Y Vengrenyuk, TJ Barrett, MZ Dewan, L Guo, D Fuller, AV Finn, R Virmani, H Li, RA Friedman, EA Fisher, R Ramasamy, AM Schmidt
JCI Insight, 2020-07-09;5(13):.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot -
Fructose liquid and solid formulations differently affect gut integrity, microbiota composition and related liver toxicity: a comparative in vivo study
Authors: R Mastrocola, I Ferrocino, E Liberto, F Chiazza, AS Cento, D Collotta, G Querio, D Nigro, V Bitonto, JC Cutrin, K Rantsiou, M Durante, E Masini, M Aragno, C Cordero, L Cocolin, M Collino
J. Nutr. Biochem., 2018-02-13;55(0):185-199.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
The Major Chromophore Arising from Glucose Degradation and Oxidative Stress Occurrence during Lens Proteins Glycation Induced by Glucose
Authors: F Ávila, G Schmeda-Hi, E Silva
Molecules, 2017-12-22;23(1):.
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western Blot -
Generation and characterization of mouse knockout for glyoxalase 1
Authors: S Jang, DM Kwon, K Kwon, C Park
Biochem. Biophys. Res. Commun., 2017-06-13;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle.
Authors: Mastrocola, Raffaell, Collino, Massimo, Nigro, Debora, Chiazza, Fausto, D'Antona, Giuseppe, Aragno, Manuela, Minetto, Marco A
PLoS ONE, 2015-03-09;10(3):e0119587.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Increased protein aggregation in Zucker diabetic fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfide.
Authors: Talaei, Fatemeh, Van Praag, Veroniek, Shishavan, Mahdi H, Landheer, Sjoerd W, Buikema, Henk, Henning, Robert H
BMC Cell Biol, 2014-01-06;15(0):1.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
An advanced glycation end product (AGE)-receptor for AGEs (RAGE) axis restores adipogenic potential of senescent preadipocytes through modulation of p53 protein function.
Authors: Chen, Chih-Yu, Abell, Allison, Moon, Yang Soo, Kim, Kee-Hong
J Biol Chem, 2012-11-13;287(53):44498-507.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Advanced glycation end products accumulate in vascular smooth muscle and modify vascular but not ventricular properties in elderly hypertensive canines.
Authors: Shapiro BP, Owan TE, Mohammed SF, Meyer DM, Mills LD, Schalkwijk CG, Redfield MM
Circulation, 2008-08-18;118(10):1002-10.
Species: Canine
Sample Types: Tissue Homogenates
Applications: Western Blot -
Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice
Authors: E Benetti, R Mastrocola, F Chiazza, D Nigro, G D'Antona, V Bordano, R Fantozzi, M Aragno, M Collino, MA Minetto
PLoS ONE, 2018-01-17;13(1):e0189707.
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Mitigation of diabetes-related complications in implanted collagen and elastin scaffolds using matrix-binding polyphenol
Authors: James P. Chow, Dan T. Simionescu, Harleigh Warner, Bo Wang, Sourav S. Patnaik, Jun Liao et al.
Biomaterials
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