Acridine Orange hydrochloride

Biological Activity

Used for: cell cycle and apoptosis determination, for visual detection of nucleic acids on agarose and polyacrylamide gels, for autophagy analyze.

Application: suitable for flow cytometry and fluorescence microscopy.

Properties and Photophysical Data: emits green fluorescence when incorporated into double-stranded DNA, red fluorescence when bound electrostatically to phosphate groups of RNA or single-stranded DNA, and orange fluorescence in acidic conditions. Excitation and emission maxima (λ) are 502 nm and 525 nm, respectively (green, double strands)/ 460 nm and 650 nm, respectively (red, single strands)/ and 475 nm and 590 nm, respectively (orange, acidic conditions).

This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression.
   Kiyoshima et al.
   PLoS ONE, 2013;8:80998
2. Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
   McMaster et al.
   Proc.Natl.Acad.Sci.USA, 1977;74:4835
3. Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons.
   Ratan et al.
   J.Neurobiol., 2008;62:376

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