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| View Full Assay Principle |
The Universal Sulfotransferase Activity Kit (Catalog # EA003) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sulfotransferases that use 3-phosphoadenosine-5-phosphosulfate (PAPS) as the donor substrate. This kit utilizes inositol monophosphatase 3 (IMPAD1) as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide 3-phosphoadenosine-5-phosphate (PAP) that is generated during sulfotransferase reactions.
Features
- Applicable for all sulfotransferases that use PAPS as the donor substrate
- Non-radioactive
- No time-consuming separation steps (e.g. column chromatography)
- Provides accurate kinetic analysis of sulfotransferase reactions
- Amenable to high-throughput analysis
Kit Contents
- CHO cell-expressed Recombinant Mouse IMPAD1
- PAP
- Phosphatase Buffer 3
- Phosphate Standard
- Malachite Green Reagents A & B
Data Example
Activity of CHST7
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The ability of Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST) to transfer sulfate from PAPS to N-acetyl glucosamine was measured using the Universal Sulfotransferase Activity Kit (Catalog # EA003). The corrected optical densities were plotted versus the amount of enzyme. Specific activity of CHST7 was calculated to be 116.4 pmol/min/μg. |