Buffer Group 1
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20 | 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 | 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 2
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20 | 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 | 2% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 3
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20 | 2% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 | 2% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 4
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20 | 2% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 | 1% nonfat dry milk in blotting buffer, Adjust pH to 7.4 |
Buffer Group 5
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.5 M NaCl, 0.1% Tween 20 | 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 | 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 6
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.5 M NaCl, 0.1% Tween 20 | 5% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 | 2% nonfat dry milk in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 7
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.05% Tween 20 | 3% BSA in Blotting Buffer, Adjust pH to 7.4 | 0.5% BSA in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 8
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.5 M NaCl, 0.05% Tween 20 | 3% BSA in Blotting Buffer, Adjust pH to 7.4 | 0.5% BSA in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 9
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 50 mM Tris, pH 7.4, 0.5 M NaCl, 0.05% Tween 20 | 3% BSA in Blotting Buffer, Adjust pH to 7.4 | 1% BSA in Blotting Buffer, Adjust pH to 7.4 |
Buffer Group 10
| Blotting Buffer | Blocking Solution | Antibody Solution |
| 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20 | 5% BSA in Blotting Buffer, Adjust pH to 7.4 | 5% BSA in Blotting Buffer, Adjust pH to 7.4 |