Recombinant Mouse Syndecan-1 Protein, CF

Catalog #: 3190-SD
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3190-SD-050

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All Syndecan-1/CD138 Proteins and Enzymes

Product Details

Citations (5)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Syndecan-1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to enhance the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. When 5 x 10⁴cells/well are added to mouse Syndecan-1 and Recombinant Human Fibronectin (0.5 μg/mL, Catalog # 4305-FNB) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀for this effect is 2.5‑10 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Syndecan-1/CD138 protein
Gln18-Glu252, with a C-terminal 6-His tag

Accession #

P18828

N-terminal Sequence
Analysis

No results obtained: Gln18 predicted

Predicted Molecular Mass

25.3 kDa

SDS-PAGE

60-80 kDa, reducing conditions

Product Datasheets

3190-SD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3190-SD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Syndecan-1/CD138

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the Syndecan family of Type 1 transmembrane proteins (1, 2). The four Syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 22 aa signal sequence, a 233 aa extracellular domain (ECD) that includes three closely spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 21 aa TM segment, and a 35 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site (4). The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Mouse Syndecan-1 ECD shares 70% and 87% aa identity with the ECD of human and rat Syndecan-1, respectively. Alternative splicing in mouse generates an isoform with an internal deletion of 44 aa from the ECD (5). Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (6, 7). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (6 - 10). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (7, 9, 11). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (12 - 14). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (14, 15). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (6).

References

Tkachenko, E. et al. (2005) Circ. Res. 96:488.
Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
Saunders, S. et al. (1989) J. Cell Biol. 108:1547.
Romaris, M. et al. (1999) J. Biol. Chem. 274:18667.
Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
Vanhoutte, D. et al. (2007) Circulation 115:475.
Li, Q. et al. (2002) Cell 111:635.
Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
Derksen, P.W.B. et al. (2002) Blood 99:1405.
Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Entrez Gene IDs

6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

Alternate Names

CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1

Related Research Areas

Citations for Recombinant Mouse Syndecan-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Identification of the growth cone as a probe and driver of neuronal migration in the injured brain
Authors: Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors
Authors: Takeda, K;Kaifu, T;Michihata, R;Kinugawa, N;Fujioka, A;Tateno, A;Toshima, K;Kanoh, H;Inamori, KI;Kamijo, K;Himeda, T;Ohara, Y;Inokuchi, JI;Nakamura, A;
European journal of immunology
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons
Authors: Andries, L;Kancheva, D;Masin, L;Scheyltjens, I;Van Hove, H;De Vlaminck, K;Bergmans, S;Claes, M;De Groef, L;Moons, L;Movahedi, K;
Acta neuropathologica communications
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus
Authors: L Liu, M Akkoyunlu
The Journal of Biological Chemistry, 2021-08-06;0(0):101053.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Serum developmental endothelial locus-1 is associated with severity of sepsis in animals and humans
Authors: WY Kim, SH Lee, DY Kim, HJ Ryu, GR Chon, YY Park, Y Fu, JW Huh, CM Lim, Y Koh, EY Choi, SB Hong
Sci Rep, 2019-09-10;9(1):13005.
Species: Mouse
Sample Types: Protein
Applications: Bioassay