Human WISP‑1/CCN4 Biotinylated Antibody

Catalog #: BAF1627 Datasheet / COA / SDS

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BAF1627

All WISP-1/CCN4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human WISP‑1/CCN4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human WISP-1 in Western blots. In this format, approximately 50% cross‑reactivity with recombinant mouse (rm) WISP‑1 is observed and less than 1% cross‑reactivity with rhNOV and rhCTGF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WISP-1
Thr23-Asn367
Accession # O95388

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human WISP‑1/CCN4 (Catalog # 1627-WS)

Human WISP-1/CCN4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human WISP‑1/CCN4 Antibody (Catalog # MAB16271)

Standard: Recombinant Human WISP-1/CCN4 Protein, CF (Catalog # 1627-WS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WISP-1/CCN4

Human WISP-1 (Wnt-induced secreted protein-1; also CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑5). It is synthesized as a 367 aa precursor that contains a series of structural homology modules. Following a 22 amino acid (aa) signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 57 aa von Willebrand factor type C (VWC) module (aa 126‑182), a 40 aa TSP type I domain (aa 220‑259) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). At least five alternative splice forms are known for WISP-1. One is 30 kDa in size, 258 aa in length, and shows a substitution of a His for aa 95‑182. This removes the VWC domain (2, 6). A second isoform is 155 aa in length and shows a frameshift at Arg 117 with a unique 38 aa C‑terminal extension. A third is 195 aa in length and shows a 31 aa substitution for the first 203 aa of the full length precursor (6). This retains the VWC and cysteine knot domains. A fourth shows a 43 aa substitution for aa 117‑367 for a total length of 163 aa. This effectively removes everything but the IGFBP-like domain (7). The last splice form contains a deletion of aa 25‑269 for a total length of 122 aa. Thus, only the signal sequence and cysteine knot motifs are retained (8). This leaves only the IGFBP-like domain (9). Full-length mature human WISP-1 is 85% aa identical to both mouse and rat WISP-1. WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation (10, 11).

References

Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
Tanada, S. et al. (2001) Oncogene 20:5525.
Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
Brigstock, D.R. (2003) J. Endocrinol. 178:169.
Li, Z. (2005) GenBank Accession # AAP43925.
Li, Z. (2005) GenBank Accession # AAP43926.
Li, Z. (2005) GenBank Accession # AAP43924.
Cervello, M. et al. (2004) Ann. N.Y. Acad. Sci. 1028:432.
French, D.M. et al. (2004), Am. J. Pathol. 165:855.
Parisi, M.S. et al. (2006) Bone 38:671.

Long Name

Wnt-1 Induced Secreted Protein 1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)

Alternate Names

CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1