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Human/Mouse NeuroD1 Antibody

Catalog #: AF2746
18 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2746
AF2746-SP
NeuroD1 in beta TC-6 Mouse Cell Line.
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Citations (18)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NeuroD1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NeuroD1
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry NeuroD1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

NeuroD1 in beta TC-6 Mouse Cell Line. Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NeuroD1

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Long Name
Neurogenic Differentiation Factor 1
Entrez Gene IDs
4760 (Human)
Alternate Names
basic helix-loop-helix transcription factor; BETA2; BHF-1; BHLHA3; bHLHa3beta-cell E-box transactivator 2; Class A basic helix-loop-helix protein 3; MODY6; NeuroD; NeuroD1; neurogenic differentiation 1; neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; NIDDM

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Citations for Human/Mouse NeuroD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie the Allan-Herndon-Dudley syndrome
    Authors: Salas-Lucia, F;Escamilla, S;Bianco, AC;Dumitrescu, A;Refetoff, S;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. CD20 is a mammalian odorant receptor expressed in a subset of olfactory sensory neurons that mediates innate avoidance of predators
    Authors: Jiang, HC;Park, SJ;Wang, IH;Bear, DM;Nowlan, A;Greer, PL;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system
    Authors: Shenghui Liang, Jia Zhao, Robert K. Baker, Elisa Tran, Lisa Zhan, Timothy J. Kieffer
    Cell Reports Methods
  4. Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents
    Authors: Raghu Ram Katreddi, Ed Zandro M. Taroc, Sawyer M. Hicks, Jennifer M. Lin, Shuting Liu, Mengqing Xiang et al.
    Development
  5. Progenitor cells from brown adipose tissue undergo neurogenic differentiation
    Authors: M Jumabay, L Zhang, J Yao, KI Boström
    Scientific Reports, 2022-04-04;12(1):5614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats
    Authors: NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon
    Frontiers in Neuroscience, 2021-09-14;15(0):689601.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells
    Authors: H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be
    Nature Communications, 2021-06-07;12(1):3330.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  8. Deletion of Btg1 Induces Prmt1-Dependent Apoptosis and Increased Stemness in Shh-Type Medulloblastoma Cells Without Affecting Tumor Frequency
    Authors: Manuela Ceccarelli, Giorgio D'Andrea, Laura Micheli, Felice Tirone
    Frontiers in Oncology
  9. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020-02-24;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  10. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020-02-17;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: ICC
  11. Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development
    Authors: N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton
    Cell Rep, 2019-05-21;27(8):2281-2291.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets
    Authors: Nadav Sharon, Raghav Chawla, Jonas Mueller, Jordan Vanderhooft, Luke James Whitehorn, Benjamin Rosenthal et al.
    Cell
  13. The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging
    Authors: Kevin M. Child, Daniel B. Herrick, James E. Schwob, Eric H. Holbrook, Woochan Jang
    The Journal of Neuroscience
  14. Tis21-gene therapy inhibits medulloblastoma growth in a murine allograft model
    Authors: Dario Presutti, Manuela Ceccarelli, Laura Micheli, Giuliana Papoff, Simonetta Santini, Simone Samperna et al.
    PLOS ONE
  15. Terminal Differentiation of Adult Hippocampal Progenitor Cells Is a Step Functionally Dissociable from Proliferation and Is Controlled by Tis21, Id3 and NeuroD2
    Authors: Laura Micheli, Manuela Ceccarelli, Roberta Gioia, Giorgio D’Andrea, Stefano Farioli-Vecchioli, Marco Costanzi et al.
    Frontiers in Cellular Neuroscience
  16. Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells.
    Authors: Cechin S, Alvarez-Cubela S, Giraldo J, Molano R, Villate S, Ricordi C, Pileggi A, Inverardi L, Fraker C, Dominguez-Bendala J
    Stem Cells Transl Med, 2013-12-27;3(3):277-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  17. Tis21 knock-out enhances the frequency of medulloblastoma in Patched1 heterozygous mice by inhibiting the Cxcl3-dependent migration of cerebellar neurons
    Authors: Stefano Farioli-Vecchioli, Irene Cinà, Manuela Ceccarelli, Laura Micheli, Luca Leonardi, Maria Teresa Ciotti et al.
    The Journal of Neuroscience
  18. Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3.
    Authors: Farioli-Vecchioli S, Tanori M, Micheli L, Mancuso M, Leonardi L, Saran A, Ciotti MT, Ferretti E, Gulino A, Pazzaglia S, Tirone F
    FASEB J., 2007-03-19;21(9):2215-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human/Mouse NeuroD1 Antibody
By Anonymous on 06/08/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: olfactory epithelium Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse NeuroD1 Antibody AF2746